溶血磷脂酸协同TGFβ1促进结肠癌细胞上皮间质转化的研究  

The effect and mechanism of lysophosphatidic acid oil metastasis and anti-apoptosis in colorectal carcinoma

作　　者：邓镇平 高旭灿[2] 韦智经 邱际亮 许鹏 刘海鹰 

机构地区：[1]广州市荔湾区第二人民医院普外科,510160 [2]深圳市人民医院肛肠外科,518000 [3]广州医科大学附属肿瘤医院胃肠肿瘤外科,510000

出　　处：《中华结直肠疾病电子杂志》2015年第4期44-46,共3页Chinese Journal of Colorectal Diseases(Electronic Edition)

基　　金：广东省科技厅科研基金(210B080702001)

摘　　要：目的探讨溶血磷脂酸(LPA)协同转化生长因子1(TGFβ1)的促上皮间质转化(EMT)作用。方法观察TGFβ1及LPA诱导SW480细胞后细胞形态变化,Western blotting方法检测E-Cadherin、Vimentin的表达变化以及细胞增殖活性变化。结果 TGFβ1及LPA均能使SW480细胞形态发生EMT改变;两者联合诱导细胞EMT改变更为明显。空白对照组、LPA组、TGFβ1组、LPA组+TGFβ1组,E-Cadherin表达依次下降;Vimentin表达未见明显差异。结论 LPA可能协同TGF-β1产生EMT作用,下调LPA的表达或可抑制肿瘤侵袭转移。Objective This study aimed to investigate whether LPA could synergize TGFβ1 to increase epithelial-to-mesenchymal transition(EMT).Methods Western blotting was used to examine the EMT markers:E-cadherin and Vimentin .MTT was used to examine the cell proliferation of 5-Fu and LOHP with or without LPA.Western blotting was used to examine the apoptosis markers Bcl-2,Bax and Caspese-3 after LPA treatment .Results TGFβ1 and LPA induced the morphology change of colorectal cancer cell line SW480 , and combination group LPA/TGFβ1 induced more significant change .Compared with the LPA group,the difference of E-cadherin in blank control group 1%FBS,and TGFβ1 treatment group,the LPA/TGFβ1 co-treatment group were significant ,while the difference of Vimentin was not significant .Conclusion LPA synergizes with TGF-βto play an important role in EMT .Inhibition of LPA may decrease the invasion and migration of colon cancer cells .

关 键 词：溶血磷脂酸类 结直肠肿瘤 协同转化生长因子1 

分 类 号：R735.35[医药卫生—肿瘤]