Inhibitory effect of polypeptides from Chlamys farreri on UVB-induced apoptosis and DNA damage in normal human dermal fibroblasts in vitro  

扇贝多肽对紫外线B所致人真皮成纤维细胞凋亡和DNA损伤的抑制作用(英文)

作　　者：丁擘晓[1] 王春波[1] 

机构地区：[1]青岛大学医学院药理教研室

出　　处：《Acta Pharmacologica Sinica》2003年第10期1006-1010,1061,1062,共7页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (№ 39970638); the Science and Technology Bureau of Qingdao (№ 2001-28-50).

摘　　要：AIM: To investigate the effect of polypeptide from Chlamys farreri(PCF) on ultraviolet B(UVB)-induced apoptosis and DNA damage in cultured normal human dermal fibroblasts. METHODS: MTT assay was used to measure the viability of cells. Measurements of apoptosis and cytosolic free[Ca^(2+)]_i were performed with flow cytometry. The comet assay was employed to detect DNA damage in individual cell. RESULTS: PCF (0.25%-1%) greatly enhanced the proliferative capacity of cultured fibroblasts irradiated by UVB(1.176×10^(-4) J-cm^(-2)) and markedly reduced apoptosis and the level of DNA damage in a concentration-dependent manner. Meanwhile, PCF could decrease the cytosolic free[Ca^(2+)]_i (P<0.01, compared with UVB model). CONCLUSION: The inhibitory effect of PCF on UVB-induced photoaging is due to enhanced abating of UVB-injured DNA and UVB-induced apoptosis. Therefore, PCF can resist UV-induced aging development at the initiation stage.目的:研究扇贝多肽(PCF)对中波紫外线(UVB)所致体外培养人真皮成纤维细胞的凋亡和DNA损伤的影响。方法:用噻唑兰(MTT)法检测成纤维细胞的存活率;流式细胞术测定细胞的凋亡率和胞浆内游离[Ca^(2+)]_i;彗星电泳法检测单细胞DNA受损的程度。结果:PCF(0.25％-1％)能显著提高UVB(1.176×10^(-4) J·cm^(-2))辐射后人真皮成纤维细胞的增殖活性,降低UVB所致细胞的凋亡率和死亡率,减轻受损的程度,同时可降低胞浆内游离[Ca^(2+)]_i(和UVB模型组相比,P<0.01).结论:PCF对抗UVB所致的光老化是基于其减轻UVB对DNA的损伤和降低UVB所致细胞的凋亡,因此,PCF能够在起始阶段阻止紫外线所致光老化的发展。

关 键 词：Chlamys farreri ultraviolet rays apoptosis DNA damage FIBROBLASTS 

分 类 号：R96[医药卫生—药理学]