miR-205过表达对胶质母细胞瘤U-87细胞生物学行为的影响  

Effects of miR-205 over-expression on biological behaviors in glioblastoma U-87 cells

作　　者：陈枉枉 房建凯[2] 孙莉[1] 孙茂民 

机构地区：[1]苏州大学医学部解剖学和细胞神经生物学研究室,江苏苏州215123 [2]苏州大学医学部病理研究室,江苏苏州215123 [3]苏州大学医学部实验动物中心,江苏苏州215123

出　　处：《江苏大学学报（医学版）》2015年第5期390-394,共5页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:研究微小RNA-205(miR-205)在胶质瘤细胞株中的表达,探讨miR-205对人脑星形胶质母细胞瘤细胞U-87增殖、凋亡、迁移和侵袭的影响。方法:通过实时荧光定量PCR(qRT-PCR)检测4种胶质瘤细胞株(U251、A172、U-118和U-87)中miR-205的表达;利用miR-205 mimic瞬时转染U-87细胞,qRT-PCR检测转染后细胞中miR-205的表达水平;采用MTT法检测U-87细胞的增殖能力;流式细胞术检测U-87细胞的凋亡;划痕实验检测U-87细胞的迁移能力;Transwell实验检测U-87细胞的侵袭能力。结果:miR-205在胶质瘤细胞株中的表达较正常胶质细胞株低,miR-205 mimic显著上调U-87细胞中miR-205的表达水平(t=9.991,P<0.01),显著抑制U-87细胞的增殖(P<0.01),显著促进U-87细胞的凋亡(t=3.185,P<0.05)。而且miR-205 mimic还可以显著抑制U-87细胞的迁移(t=4.425,P<0.05)和侵袭能力(t=11.81,P<0.01)。结论:miR-205在胶质瘤细胞株中低表达,过表达miR-205能够有效抑制U-87细胞的增殖,促进细胞凋亡,并且抑制了细胞的迁移和侵袭能力。提示低表达的miR-205在胶质母细胞瘤的发生发展中起着重要作用,并且可能成为治疗胶质母细胞瘤的分子靶点。Objective:To investigate the expression of microRNA-205 (miR-205 )in the glioma cell strains and the impact of miR-205 on proliferation,apoptosis,migration and invasion of human astrocyte glioblastoma cells U-87.Methods:Real-time quantitative PCR (qRT-PCR)was applied to detect the ex-pression of miR-205 in 4 kinds of glioma cell strains.miR-205 mimic was transiently transfected into U-87 cells,and then the expression of miR-205 was detect by qRT-PCR.MTT assay was used to detect the pro-liferation ability of U-87 cells;apoptosis of U-87 cells was measured by flow cytometry.Wound healing as-say and transwell assay was used to detect the migration and invasion ability of U-87 cells.Results:miR-205 was low-expressed in glioma cell strains as compared with normal glial cells.miRNA-205 mimic signifi-cantly increased the expression of miR-205 in U-87 cells(t =9.991,P <0.01),significantly inhibited cell proliferation(P <0.01),and significantly promoted cell apoptosis(t =3.185,P <0.05).Moreover,miR-NA-205 mimic significantly inhibited the migration(t =4.425,P <0.05)and invasion(t =11.81,P <0.01)ability of U-87 cells.Conclusion:miR-205 was low-expressed in glioma cell strains.The over-ex-pression of miR-205 can reduce proliferation,increase apoptosis,and inhibit migration and invasion in U-87 cells.miRNA-205 might play an important role in the progress of glioblastoma and become a new molecu-lar target for the treatment of glioblastoma.

关 键 词：胶质母细胞瘤 微小RNA-205 增殖 凋亡 迁移 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]