幽门螺杆菌感染者胃黏膜中内质网分子伴侣Grp94的表达  

Expression of glucose-regulation protein 94 in gastric mucosa infected with Helicobacter pylori

作　　者：王孟春[1] 方文刚[2] 顾金歌[3] 李岩[4] 

机构地区：[1]中国医科大学附属二院内镜中心,辽宁省沈阳市110004 [2]中国医科大学发育生物学研究室,辽宁省沈阳市110001 [3]沈阳市红十字会医院消化内科,辽宁省沈阳市110014 [4]中国医科大学第二临床学院消化内科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2003年第5期551-553,共3页World Chinese Journal of Digestology

摘　　要：目的:探讨幽门螺杆菌感染对胃黏膜组织中内质网分子伴侣Grp94mRNA及蛋白水平表达的影响及意义.方法:应用半定量RT-PCR方法及Westernblot方法检测感染与未感染幽门螺杆菌的胃黏膜组织中Grp94mRNA及蛋白的表达情况.结果:未感染组28例,Grp94mRNA表达量为0.424±0.055,感染组32例,Grp94mRNA表达量为0.882±0.082.两组相比感染组Grp94mRNA的表达量明显高于未感染组(P<0.01);未感染组Grp94蛋白表达量为0.427±0.036,感染组Grp94蛋白表达量为0.671±0.072,两组相比感染组Grp94蛋白表达量明显高于未感染组(P<0.01).结论:幽门螺杆菌感染可使胃黏膜增加Grp94mRNA的表达及蛋白的合成,这可能有利于胃黏膜的自身保护作用.AIM:To study the expression of glucose regulation protein 94 in gastric mucosa infected with Helicobacter pylori. METHODS:Semi-quantitative RT-PCR method was used to demonstrate mRNA expression of Grp94 in H.pylori (Hp) infected and non-infected gastric mucosa. Western blot was used to detect the expression level of Grp94 protein in the tissues. RESULTS:Hp negative group had 28 cases, and the expression amount of Grp94 mRNA was 0.424±0.055. Hp infected group had 32 cases and the expression amount of Grp94 mRNA was 0.882±0.082. The expression amounts of Grp94 protein were 0.427±0.036, 0.671±0.072 respectively in Hp-negative and positive groups. The expression amounts of Grp94 mRNA and Grp94 protein in infected group showed a significant increase (P <0.01) respectively. CONCLUSION:H.pylori infection may increase the expression of Grp94 at mRNA level in gastric mucosa. Meanwhile, it also increases synthesis of the protein.

关 键 词：幽门螺杆菌 胃黏膜 GRP94 半定量RT-PCR Western BLOT法 上皮细胞 

分 类 号：R573[医药卫生—消化系统]