抗癌药物对人喉鳞状细胞癌Hep-2细胞端粒酶活性的影响  

Effect of Anticancer Drug on Telomerase Activity of Human Laryngeal Carcinoma Cell Hep-2

作　　者：庞荣清[1] 刘春生[2] 吴秀琴[3] 张悦[4] 潘兴华[1] 陈志龙[1] 

机构地区：[1]成都军区昆明总医院医学实验科,云南昆明650032 [2]云南省第一人民医院肿瘤外科 [3]成都军区昆明温泉疗养院药剂科 [4]成都军区昆明总医院化验科

出　　处：《中国医师杂志》2003年第7期879-880,共2页Journal of Chinese Physician

摘　　要：目的　观察羟基喜树碱、顺铂和环磷酰胺三种抗癌药对Hep -2细胞端粒酶活性的影响。方法　运用MTT法测定 72h的药物IC50 ,在此基础上 ,运用端粒重复序列扩增 -酶联免疫吸附法测定细胞经过不同浓度的抗癌药作用不同时间后端粒酶活性的变化。结果　羟基喜树碱和环磷酰胺均能抑制Hep -2细胞增殖生长并下调端粒酶活性 ,而顺铂则促进Hep -2细胞增殖生长并上调端粒酶活性。结论　羟基喜树碱和环磷酰胺都能通过直接或间接的方式下调端粒酶活性 ,其机制可能与药物浓度及时间有关。顺铂则上调端粒酶活性 。Objective To investigate effect on telomerase activity of Hep-2 cells treated by anticancer drugs(hydroxycamptothecine, cisplatin and cytoxan).Methods By MTT method,we measured the 50% inhibitory concentration(IC 50 ) at 72h,and compared to untreated control cells. Telomerase activity of Hep-2 cells treated by the drugs in different concentration based on IC 50 for different time was observed by Telomeric Repeat Amplification Protocol with ELISA(TRAP-ELISA).Results Hydroxycamptothecine and cytoxan could inhibit proliferation of Hep-2 and down-regulate telomerase activity of Hep-2 cell. However, cisplatin promoted proliferation of Hep-2 and up-regulated telomerase activity of Hep-2 cell.Conclusions Hydroxycamptothecine and cytoxan could down-regulate telomerase activity of Hep-2 cell by direct or indirect pattern, which may correlate with drug concentration and time-dependent pattern.Cisplatin could up-regulate telomerase activity of Hep-2 cell, which mechanism is not clear.

关 键 词：抗癌药物 端粒酶活性 HEP-2细胞 酶活性 人喉鳞状细胞癌 羟基喜树碱 顺铂 环磷酰胺 

分 类 号：R739.62[医药卫生—肿瘤]