沉默I2PP2A基因对胃癌BGC-823细胞增殖和迁移能力的影响  

Effect of Down-regulated I2PP2A Expression on Proliferation and Migration of Gastric Carcinoma Cell Line BGC-823

作　　者：张荣[1] 姜涛[1] 龙炽慧 孙贝贝[1] 鄢志雄[1] 师海蓉[2] 

机构地区：[1]江汉大学医学院临床医学系,武汉430056 [2]江汉大学医学院病理学与病理生理学教研室,武汉430056

出　　处：《肿瘤防治研究》2015年第3期233-237,共5页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(81001082);武汉市教育局教研课题(2011010);江汉大学大学生学术科技活动专项基金项目(2012zd15)

摘　　要：目的探讨siRNA沉默I2PP2A基因表达对人胃癌BGC-823细胞增殖和迁移的影响及其可能的分子机制。方法脂质体转染法将靶向I2PP2A的短发夹状RNA(short hairpin RNA,shRNA)转染BGC-823细胞;Real-time RT-PCR和Western blot观察转染后胃癌BGC-823细胞I2PP2A mRNA和蛋白表达的变化;WST-1法和Transwell小室分别检测各组细胞增殖和细胞迁移能力的变化;最后采用蛋白质印迹分析法检测三组胃癌细胞中cyclin D1和MMP-9蛋白的表达。结果转染I2PP2A siRNA后胃癌BGC-823细胞的I2PP2A mRNA及蛋白表达明显受抑制;I2PP2A mRNA和蛋白表达下调抑制胃癌BGC-823细胞的增殖和迁移,并降低cyclin D1和MMP-9蛋白的表达。结论 I2PP2A表达下调介导的胃癌细胞增殖抑制和迁移降低可能与cyclin D1和MMP-9表达下调密切相关,I2PP2A可能成为胃癌治疗的新靶点之一。Objective To investigate the inhibitory effect of synthetic I2PP2A short interfering RNA(si RNA) on the proliferation and migration of human gastric cancer cell line BGC-823 and to explore the possible molecular mechanisms. Methods The si RNA targeted I2PP2A sh RNA was transfected into BGC-823 cells by LipofectamineTM 2000. Expression of I2PP2A m RNA and protein were examined by Real-time RT-PCR and Western blot, respectively. WST-1 and Transwell chamber were used to observe the proliferation and migration of BGC-823 cells, respectively. The expression of cyclin D1 and MMP-9 protein were also detected by Western blot. Results I2PP2A si RNA significantly inhibited the expression of I2PP2A in gastric cancer cell line BGC-823 at both m RNA and protein levels. Downregulated I2PP2A expression greatly inhibited the proliferation and migration of BGC-823 cells and also down-regulated the expression of cyclin D1 and MMP-9 proteins. Conclusion Down-regulatedI2PP2A-expression-mediated inhibition on the proliferation and migration of gastric carcinoma cells may be closely associated with the decreased expression of cyclin D1 and MMP-9 proteins, indicating a new approach of gene therapy for gastric cancer.

关 键 词：蛋白磷酸酶2A抑制剂-2 小分子干扰RNA 胃癌 细胞增殖 细胞迁移 

分 类 号：R735.2[医药卫生—肿瘤]