灵芝多糖对T24荷瘤裸鼠化疗效果及其免疫逃逸的影响  

Effects of Ganoderma Lucidum Polysaccharide on Chemotherapy Effect and Immune Escape in T24 Bearing Mice

作　　者：刘奔[1] 郭鹏荣 盛玉文[1] 傅德望[1] 

机构地区：[1]辽宁医学院附属第一医院泌尿外科,锦州121001

出　　处：《肿瘤防治研究》2015年第5期459-465,共7页Cancer Research on Prevention and Treatment

基　　金：辽宁省自然科学基金(201202131)

摘　　要：目的探讨灵芝多糖(ganoderma lucidum polysaccharide,GLP)对膀胱癌化疗效果的影响及其机制。方法建立T24荷瘤裸鼠模型,分组给药后测定脾脏指数,利用流式细胞仪检测小鼠脾脏和血液中相关淋巴细胞亚群所占比例,采用双抗体夹心(ELISA)法检测荷瘤小鼠血清IL-10、VEGF、TGF-β、IL-2和TNF-α含量,应用反转录-聚合酶链反应(RT-PCR)和Western blot检测小鼠脾脏IL-2和TNF-α的表达。结果在荷瘤裸鼠模型中,灵芝多糖(200 mg/kg)灌胃18 d后可显著增加脾脏指数(P<0.001);顺铂+灵芝多糖组中小鼠脾脏及血液中的淋巴细胞(CD4、CD8、NK及DC)都较顺铂组提高,而髓源性抑制细胞(myeloid derived suppressor cell,MDSC)含量降低。通过ELISA检测发现灵芝多糖能明显增加外周血液中相关免疫因子(IL-10、VEGF、TGF-β、IL-2和TNF-α)的含量(P<0.05)。RT-PCR及Western blot的结果也证实了在灵芝多糖可增强小鼠化疗后脾脏中IL-2和TNF-α的表达。结论灵芝多糖能通过提升脾指数,增加脾脏和外周血相关淋巴细胞及免疫因子的含量,并通过增强血清中IL-2和TNF-α的表达来提高小鼠的免疫力,增强抗肿瘤作用,其机制可能与灵芝多糖降低免疫逃逸功能相关。Objective To explore the effects of ganoderma lucidum polysaccharide(GLP) in chemotherapy on urinary bladder cancer and the mechanism of its role in immune escape. Methods The spleen indexes were examined in T24 bearing mice after receiving treatment with GLP and cisplatin; flow cytometry was used to test the proportion of lymphocyte subsets in spleen and blood; the serum levels of IL-10, VEGF, TGF-β, IL-2 and TNF-α were detected by ELISA. And the expression of IL-2 and TNF-α in spleen were assayed by reverse transcriptase PCR(RT-PCR) and Western blot. Results The spleen index was significantly increased after treated with GLP(200mg/kg) for 18d(P<0.001). The mice receiving cisplatin plus GLP achieved higher proportion of lymphocyte subsets(CD4, CD8, NK and DC) and lower amount of myeloid-derived suppressor cell(MDSC) in either spleen or blood, compared with those received cisplatin alone. ELISA analysis showed that GLP could obviously increase the productions of immune-related factors(IL-10, VEGF, TGF-β, IL-2 and TNF-α) in peripheral blood(P<0.05). RT-PCR and Western blot also confirmed that GLP obviously promoted the expression of IL-2 and TNF-α in spleen during chemotherapy. Conclusion GLP could enhance the immunity of mice and the anti-tumor effect of chemotherapy by improving the spleen index, increasing the contents of lymphocytes and immune factors in spleen and peripheral blood and enhancing the IL-2 and TNF-α expression in splenocytes. The mechanism may be associated with decreasing immune escape function of GLP.

关 键 词：灵芝多糖 顺铂 膀胱肿瘤 免疫因子 免疫逃逸 

分 类 号：R737.14[医药卫生—肿瘤]