硒蛋白S基因G-105A、G-254A位点多态性与湖南汉族人群胃癌遗传易感性的相关研究  

Association of Selenoprotein SG-105A,G-254A Polymorphisms with Genetic Susceptibility to Gastric Cancer in Hu'nan Han Population

作　　者：毛华杰[1] 朱燕[1] 卢淑华[1] 李诚城 江昊宇 黄玉梅[2] 章迪[3] 王晓春[1] 

机构地区：[1]中南大学湘雅医学院医学检验系,长沙410013 [2]湖南省肿瘤医院检验科,长沙410013 [3]中南大学湘雅三医院检验科,长沙410013

出　　处：《肿瘤防治研究》2015年第6期582-586,共5页Cancer Research on Prevention and Treatment

基　　金：中南大学研究生自主探索创新项目(2014zzts328)

摘　　要：目的探讨湖南汉族人群硒蛋白S(Sel S)基因G-105A、G-254A位点多态性与胃癌遗传易感性的相关研究。方法采用聚合酶链反应—限制性片断长度多态性(PCR-RFLP)方法,检测113例湖南汉族胃癌患者及111例健康对照者基因型和等位基因频率,分析胃癌患者与健康对照者G-105A、G-254A位点等位基因频率及基因型频率的差异。结果 G-105A位点GG、GA和AA基因型在胃癌组和健康对照组间分别为100.0%、0.0%、0.0%和100.0%、0.0%、0.0%;G、A等位基因频率在胃癌组和健康对照组分别为100.0%、0.0%和100.0%、0.0%,胃癌组和健康对照组基因型和等位基因频率分布无差异。G-254A位点CC、CT和TT基因型在胃癌和健康对照组间分别为50.0%、37.2%、12.8%和65.8%、28.8%、5.4%;C、T等位基因频率在胃癌组和健康对照组分别为69.0%、31.0%和80.2%、19.8%。胃癌组和健康对照组基因型和等位基因频率分布差异有统计学意义(P<0.05)。湖南汉族人群中携带T等位基因的个体患胃癌的风险是CC基因型个体的1.89倍(OR=1.89,9 5%C I:1.1 0~3.2 3)。结论 S e l S G-254A位点多态性与胃癌的发病具有相关性,T等位基因可能是湖南汉族人群胃癌发病的危险因素之一;湖南汉族人群可能无G-105A这一位点的多态性分布。Objective To investigate the association between selenoprotein S(Sel S) gene G-105 A,G-254 A polymorphisms and genetic susceptibility to gastric cancer in Hu'nan Han population.Methods Polymerase chain reaction restriction fragment length of polymorphism(PCR-RFLP) was taken to detect the genotypes and allele frequencies of the Sel S in 113 gastric cancer patients in Hu'nan Han population and 111 agematched healthy controls,to analyze the differences of allele and genotype frequencies of G-105 A and G-254 A.Results Genotype frequencies of GG,GA,AA in Sel S G-105 A loci were 100.0%,0.0%,0.0% in gastric cancer group,and 100.0%,0.0%,0.0% in control group respectively.Allele frequencies of G,A were 100.0%,0.0% in gastric cancer group and 100.0%,0.0% in control group respectively.There was no difference of genotype and allele frequencies of Sel S G-105 A polymorphism between gastric cancer group and control group.Genotype frequencies of CC,CT,TT in Sel S G-254 A were 50.0%,37.2%,12.8% in gastric cancer group and 65.8%,28.8%,5.4% in control group respectively.Allele frequencies of C,T were 69.0%,31.0 in gastric cancer group and 80.2%,19.8% in control group respectively.There were significant differences of genotype and allele frequencies of Sel S G-254 A polymorphism between gastric cancer group and control group(P<0.05).The relative risk of suffering from gastric cancer in Hu'nan Han population with T allele was 1.89 times of those with CC genotype(OR=1.89,95%CI:1.10-3.23).Conclusion Sel S G-254 A polymorphism is associated with the risk of gastric cancer.T allele may be a risk factor for gastric cancer in Hu'nan Han population.There may be no G-105 A polymorphism distribution in Hu'nan Han population.

关 键 词：硒蛋白S 多态性 胃癌 遗传易感性 

分 类 号：R735.2[医药卫生—肿瘤]