大肠癌中Pokemon的表达及与前哨淋巴结微转移的关系  

Relationship Between Pokemon Expression and Sentinel Lymph Node Micro-metastasis in Colorectal Cancer

作　　者：王鹏飞[1] 王涛[2] 黄晓俊[1] 王祥[1] 张德奎[1] 樊红[1] 冯彦虎[1] 王伟[1] 吴静[3,4] 

机构地区：[1]兰州大学第二医院消化科,兰州730030 [2]甘肃省人民医院普外科,兰州730000 [3]北京大学第九临床医学院 [4]北京世纪坛医院消化内科,北京100038

出　　处：《肿瘤防治研究》2015年第6期587-590,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探索大肠癌中Pokemon的表达与大肠癌前哨淋巴结微转移的关系。方法采用RT-PCR方法检测58例Dukes A-B期大肠癌组织中癌巢、癌以远组织中Pokemon m RNA的表达水平。采用免疫组织化学法检测其中56例大肠癌癌巢中Pokemon蛋白和前哨淋巴结CK20蛋白的表达。结果大肠癌癌巢中Pokemon m RNA水平显著高于癌以远组织(P=0.000)。Pokemon蛋白阳性与阴性患者前哨淋巴结发生微转移的比例间差异未达统计学意义(P=0.562)。Pokemon蛋白阳性的患者中CK20阴性的患者生存率高于CK20阳性的患者(P=0.039)。结论 Pokemon在大肠癌癌巢中高表达,联合检测Pokemon与前哨淋巴结CK20在大肠癌预后判断中有重要价值,但尚不能认为Pokemon蛋白可以影响大肠癌前哨淋巴结微转移。Objective To investigate the relationship between the expression of POK erythroid ontogenic factor(Pokemon) and sentinel lymph node micro-metastasis(SLNMM) in colorectal cancer.Methods The expression of Pokemon m RNA in the tumor and surrounding tissues were detected by reverse transcription polymerase chain reaction(RT-PCR) from 58 cases of stage Dukes A-B colorectal cancer cured by operation.The expression of Pokemon protein and sentinel lymph node cytokeratin20(CK20) protein were detected by immunohistochemical(IHC) techniques in colorectal cancer tissues.Results The expression of Pokemon m RNA in colorectal cancer tissues was markedly higher than that in the surrounding tissues(P=0.000).But the rates of micro-metastasis between the groups of positive and negative Pokemon expression had no significant difference(P=0.562),and it was shown that the survival rate of patients with Pokemon(+)CK20(-) expression was higher than that of those with Pokemon(+)CK20(+) expression(P=0.039).Conclusion Pokemon is overexpressed in colorectal cancer tissues.The combined detection of Pokemon and sentinel lymph node CK20 protein has important value in the prognosis of colorectal cancer.But we could not consider that Pokemon protein could affect SLNMM yet.

关 键 词：大肠癌 POKEMON 前哨淋巴结微转移 

分 类 号：R735.34[医药卫生—肿瘤]