miR-200c增强肺癌A549细胞对紫杉醇及吉非替尼的敏感度及相关机制  

miR-200c Enhances Sensitivity of Lung Cancer Cell A549 to Paclitaxel and Gefitinib and Related Mechanism

作　　者：胡丽丽[1] 尹燕军[1] 钟文娟[1] 邱峰[1] 

机构地区：[1]南昌大学第一附属医院肿瘤科,南昌330006

出　　处：《肿瘤防治研究》2015年第8期760-764,共5页Cancer Research on Prevention and Treatment

摘　　要：目的观察上调肺癌A549细胞中mi R-200c的表达对紫杉醇、吉非替尼敏感度及两药联合作用的影响及其作用机制。方法用mi R-200c转染肺癌A549细胞,Real-time PCR检测mi R-200c表达,MTT法和流式细胞仪检测转染mi R-200c后A549细胞对紫杉醇、吉非替尼单药及两药联合的敏感度和细胞凋亡的影响,Western blot检测TUBB3蛋白、EGFR、Akt、MAPK磷酸化蛋白及总蛋白的表达。结果上调mi R-200c表达可增强A549细胞对紫杉醇及吉非替尼敏感度并促进细胞凋亡,对两药联合无明显作用。Western blot显示转染mi R-200c联合紫杉醇可下调A549细胞中TUBB3蛋白表达,联合吉非替尼下调EGFR和Akt磷酸化蛋白表达,与两药联合对EGFR和Akt磷酸化蛋白表达无明显影响。结论mi R-200c可增强肺癌A549细胞对紫杉醇、吉非替尼的敏感度,可能分别与抑制TUBB3表达、抑制EGFR和Akt磷酸化蛋白表达有关,而对两药联合无协同抗肿瘤作用。Objective To observe the influence of up-regulating mi R-200 c expression on the sensitivity of lung cancer cells A549 to paclitaxel, gefitinib and the combination of two drugs and related mechanism. Methods mi R-200 c was transfected into lung cancer cells A549 using Lipofectamine 2000. The expression of mi R-200 c was detected by Real-time PCR. Drug sensitivity and cell apoptosis were analyzed with MTT assay and flow cytometry. Western blot was used to detect the expression of TUBB3 protein, EGFR, Akt, MAPK expression of phosphorylated proteins and total proteins. Results The sensitivity of A549 cells to paclitaxel and gefitinib and cell apoptosis were increased after up-regulation of mi R-200 c expression, but there was no obvious effect to the combination of two drugs. Western blot showed that mi R-200 c combined with paclitaxel inhibited the expression of TUBB3 protein; mi R-200 c combined with gefitinib inhibited the expression of EGFR, Akt phosphorylation protein, but there was no obvious difference when mi R-200 c combined with the combination of two drugs. Conclusion mi R-200 c could increase the sensitivity of lung cancer cells A549 to paclitaxel and gefitinib, which maybe respectively related to the inhibition of TUBB3 and EGFR, Akt phosphorylation protein, while there is no synergistic antitumor effect on the combination of two drugs.

关 键 词：MIR-200C 肺癌 紫杉醇 吉非替尼 敏感度 

分 类 号：R734.2[医药卫生—肿瘤]