LAP^+CD4^+T细胞在结直肠癌肿瘤微环境中的分布情况及其临床意义  

Distribution of LAP^+CD4^+ T cells in colorectal cancer microenvironment and its clinical significance

作　　者：钟武[1] 江志远[2] 钟世彪[2] 张磊昌[2] 黄家豪[2] 张森[2] 陈利生[2] 曹云飞[2] 

机构地区：[1]赣州市人民医院普外一科,江西赣州341000 [2]广西医科大学第一附属医院结直肠肛门外科,广西南宁530021

出　　处：《中国肿瘤生物治疗杂志》2015年第5期612-618,共7页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.81260316);广西研究生教育创新计划资助项目(No.YCBZ2014032)~~

摘　　要：目的:检测LAP+CD4+T细胞在结直肠癌患者肿瘤微环境中的分布情况,分析其与CD4+CD25+Treg及患者临床病理特征的相关性,初步探讨LAP+CD4+T细胞在结直肠癌发生、发展中的作用。方法:收集2014年1月至2014年3月期间广西医科大学第一附属医院结直肠肛门外科收治并行手术治疗的50例结直肠癌患者临床病理资料,采集所有患者术前外周血、术中肿瘤组织和距离癌组织边缘10 cm以上的相应癌旁组织标本,同时采集本科室25例健康志愿者外周血作为对照组;流式细胞术检测各标本中LAP+CD4+T细胞和CD4+CD25+Treg的分布比例,比较结直肠癌患者与健康志愿者外周血中LAP+CD4+T细胞分布比例差异、结直肠癌肿瘤组织与相应癌旁组织中LAP+CD4+T细胞分布比例差异;分析LAP+CD4+T细胞和CD4+CD25+Treg与临床病理特征的相关性。结果:50例结直肠癌患者和25位健康志愿者外周血及组织标本LAP+CD4+T细胞占CD4+T细胞比例,结直肠癌患者外周血[(9.44±3.18)%]高于正常人外周血[(1.49±1.00)%,P=0.000]、结直肠癌患者肿瘤组织[(11.76±3.74)%]高于相应癌旁组织[(3.87±1.64)%,P=0.000];其中肿瘤组织中LAP+CD4+T细胞的分布比例最高。肿瘤组织中LAP+CD4+T细胞与CD4+CD25+Treg细胞成正相关(r=0.327,P=0.02);LAP+CD4+T细胞占CD4+T细胞的比例与肿瘤的TNM分期、远处转移及CEA水平相关(P<0.05)。结论:LAP+CD4+T细胞易聚集于结直肠癌肿瘤组织中,可能参与了结直肠癌的发生、发展,起到促进肿瘤生长、转移的作用。Objective: This study aimed:( 1) to determine the distribution of LAP+CD4+T cells in the tumor microenvironment of colorectal cancer( CRC);( 2) to analyze the associations of LAP+CD4+T cells with CD4+CD25+Treg and clinicopathological variables; and( 3) to evaluate the role for LAP+CD4+T cells in CRC development and progression.Methods: Clinicopathologic data of 50 CRC patients who were hospitalized and received surgical treatment in the Colorectal and Anal Department of Guangxi Medical University-Affiliated First Hospital of between January,2014 and March,2014 were collected. Preoperative peripheral blood,intraoperative tumor tissue and corresponding para- carcinoma tissue( over 10 cm from the tumor margins) specimens were collected for all patients. Peripheral blood samples were collected from 25 healthy volunteers for the purpose of control. Proportions of LAP+CD4+T cells and CD4+CD25+Treg in all samples were determined by flow cytometry. Associations of LAP+CD4+T cells with CD4+CD25+Treg and clinicopathological variables were analyzed. Results: The proportion of LAP+CD4+T cells in the peripheral blood was significantly higher in CRC patients( 9. 44 ± 3. 18%) than in healthy controls( 1. 49 ± 1. 00%)( P = 0. 000),and was also significantly higher in tumor tissue( 11. 76 ± 3. 74%) than in the corresponding para-carcinoma tissue( 3. 87 ± 1. 64%) in CRC patients( P= 0. 000). LAP+CD4+T cells were positively correlated with CD4+CD25+Treg cells in tumor tissues( r = 0. 327,P = 0. 02) and also with TNM staging,distant metastasis and CEA levels( P < 0. 05). Conclusion: LAP+CD4+T cells are prone to aggregate in the CRC tissue,thus promoting CRC growth and metastasis.

关 键 词：LAP+CD4+T细胞 调节性T细胞 结直肠癌 肿瘤微环境 

分 类 号：R735.34[医药卫生—肿瘤]