mir-134/Pum2信号通路在匹罗卡品致颠痫大鼠脑组织中的表达及作用  

Expression and function of mir-134 / Pum2 signal pathway in pilocarpine-induced rat model

作　　者：吴旭玲 陈阳美[1] 

机构地区：[1]重庆医科大学附属第二医院神经内科,重庆400010

出　　处：《中国老年学杂志》2015年第6期1604-1606,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81171225)

摘　　要：目的探讨micro-134(mir-134)/Pumilio2(Pum2)信号通路在癫痫大鼠模型脑组织中的表达变化及意义。方法建立氯化锂-匹罗卡品大鼠癫痫模型,用荧光定量PCR检测脑组织内mir-134在癫痫大发作后不同时间点的表达水平,用蛋白免疫印迹检测Pum2蛋白在相应时间点的表达并用双标免疫荧光技术对Pum2进行定位。结果 mir-134在癫痫大发作后的急性期(1 d和3 d)和慢性期(30 d和60 d)表达水平都较对照组显著升高(P<0.05);与对照组相比,Pum2在癫痫模型中各时间点的表达水平均明显下降(P<0.05),且Pum2主要表达于神经元胞质和树突中。结论 mir-134和Pum2在癫痫模型中异常表达,可能通过调节兴奋性环路的形成参与癫痫的发生发展过程。Objective To investigate the expression levels of mir-134 and Pum2 for exploring the possible role of mir-134 /Pum2 signal pathway in epileptogenesis. Methods Lithium-pilocarpine induced rat model was established,mir-134 and Pum2 expressions was detected in experimental animals by real time quantitative PCR( RT-q PCR) and Western blot,respectively. The localization of Pum2 was detected by double-labeled immunofluorescence. Results mir-134 was significantly increased in acute stage( 1 d and 3 d) and chronic stage( 30 d and 60 d) after seizure,and Pum2 was down-regulated at all time points after seizure when compared with control group( P < 0. 05). Conclusions mir-134 / Pum2 signal pathway might participate in the process and development of epilepsy.

关 键 词：癫痫 micro-134 Pumilio2 兴奋性环路 

分 类 号：R742.1[医药卫生—神经病学与精神病学]