中红外光纤技术用于口腔肿瘤在体原位检测的研究  

In Vivo and Rapid Detection of the Malignancy Tumor of Oral Tissues Using Mid-FTIR Fiber Optics Spectroscopy

作　　者：徐怡庄[1] 张元福[1] 杨丽敏[1] 彭歆[2] 孙开华[2] 李庆波[1] 周维金[1] 武惠中 俞光岩[2] 刘智[1] 翁诗甫[1] 王晶[2] 赵莹[4] 徐端夫[4] 吴瑾光[1] 

机构地区：[1]北京大学化学与分子工程学院,稀土材料化学及应用国家重点实验室,北京100871 [2]北京大学口腔医学院,北京100081 [3]北京第二光学仪器厂,北京100015 [4]中国科学院化学研究所,北京100080

出　　处：《高等学校化学学报》2004年第2期348-350,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :3 973 0 160 ;5 0 10 3 0 0 1;2 0 1710 0 4);国家重点基础研究发展规划项目 (批准号 :G19980 613 0 7;2 0 0 2 CCA 0 190 0 )资助

摘　　要：Our previous studies show that mid-FTIR spectroscopy can be used to distinguish malignant oral tissue from normal tissue under in vitro condition. Here, an in-situ FTIR spectroscopic measurement was performed to record FTIR spectra of normal and malignant oral tissues including salivary gland, tongue, parotid gland, submandibular gland etc. during clinical examination. The FTIR spectra of various oral tissues were acquired when an ATR probe linked to the FTIR spectrometer via mid-IR optical fibers was pressed on the tissues of the patients. For example, a patient(male, 76 years old) with tumor on the left parotid and the corresponding normal tissue on the right parotid were measured and obvious differences were observed. The spectral features of normal tissue and tumor are in good agreement with the criteria established in our previous work. (1) 1 389 cm -1 band is quite strong in tumor, while the corresponding band in normal tissue is weaker than 1 452 cm -1 band. (2) In normal tissue, 1 250 cm -1 band is stronger, but the 1 250 cm -1 band disappeared in the skin of malignant tissue. The above results demonstrate that in vivo FTIR spectra are in good agreement with our previous results obtained under in vitro condition. We believe that in vivo FTIR spectroscopy, providing the first-hand information concerning whether the suspected tissue is cancerous or not, is helpful for doctors in clinical activity.Our previous studies show that mid-FTIR spectroscopy can be used to distinguish malignant oral tissue from normal tissue under in vitro condition. Here, an in-situ FTIR spectroscopic measurement was performed to record FTIR spectra of normal and malignant oral tissues including salivary gland, tongue, parotid gland, submandibular gland etc. during clinical examination. The FTIR spectra of various oral tissues were acquired when an ATR probe linked to the FTIR spectrometer via mid-IR optical fibers was pressed on the tissues of the patients. For example, a patient(male, 76 years old) with tumor on the left parotid and the corresponding normal tissue on the right parotid were measured and obvious differences were observed. The spectral features of normal tissue and tumor are in good agreement with the criteria established in our previous work. (1) 1 389 cm -1 band is quite strong in tumor, while the corresponding band in normal tissue is weaker than 1 452 cm -1 band. (2) In normal tissue, 1 250 cm -1 band is stronger, but the 1 250 cm -1 band disappeared in the skin of malignant tissue. The above results demonstrate that in vivo FTIR spectra are in good agreement with our previous results obtained under in vitro condition. We believe that in vivo FTIR spectroscopy, providing the first-hand information concerning whether the suspected tissue is cancerous or not, is helpful for doctors in clinical activity.

关 键 词：中红外光纤技术 口腔肿瘤 在体原位检测 早期诊断 振动光谱 

分 类 号：R739.8[医药卫生—肿瘤] R730.4[医药卫生—临床医学]