趋化因子受体CXCR4在鼻咽癌细胞中的表达  

Expression of Chemokine Receptor CXCR4 in Nasopharyngeal Carcinoma Cells

作　　者：徐永[1] 张素珍[2] 黄培春[2] 陈锦[2] 蔡康荣[3] 

机构地区：[1]广东医学院生理学教研室,广东湛江524023 [2]广东医学院病理生理学教研室,广东湛江524023 [3]广东医学院分析中心,广东湛江524023

出　　处：《癌症》2004年第2期136-140,共5页Chinese Journal of Cancer

摘　　要：背景与目的:研究表明,趋化因子受体CXCR4及其配体SDF-1(stromalcell-derivedfactor1)与肿瘤的增殖、分化和转移等恶性表现密切相关。本研究通过观察分化程度和增殖能力不同的鼻咽癌细胞中CXCR4的表达,初步了解CXCR4与鼻咽癌细胞恶性表现的关系。方法:分别以全反式维甲酸(all-trans-retinoicacid,RA)和端粒酶反义核酸处理鼻咽癌CNE1(高分化)和CNE2Z(低分化)细胞,原位杂交技术检测CXCR4mRNA表达,免疫组化法检测CXCR4蛋白表达,流式细胞仪检测细胞周期分布,MTT法检测细胞增殖能力。结果:CXCR4mRNA和CXCR4蛋白在鼻咽癌CNE1和CNE2Z细胞均呈强阳性表达,且CNE2Z细胞表达强度显著高于CNE1细胞。经1×10-5mol/L和1×10-4mol/LRA作用后,与对照组比较,CNE1细胞G1期细胞明显增多,CNE2Z细胞S期细胞明显增多,同时两者的CXCR4mRNA表达水平均显著低于对照组(P<0.01),其中1×10-4mol/LRA作用较1×10-5mol/LRA作用强(P<0.01)。经端粒酶反义核酸作用后,CNE1和CNE2Z细胞增殖均明显受抑制,CXCR4蛋白表达水平均显著低于对照组(P<0.01)。结论:CXCR4在鼻咽癌细胞高表达,其表达水平与鼻咽癌细胞的分化程度和增殖能力有关。BACKGROUND &OBJECTIVE: It was reported that chemokine receptor CXCR4 and its ligand stromal cell derived factor 1(SDF 1) were involved in the proliferation, differentiation, and metastasis of tumor. This study was designed to observe the expression of CXCR4 in NPC cells with different differentiation grade and proliferative ability to primitively clarify the relationship between CXCR4 and the malignity of NPC cells. METHODS: After treated with all trans retinoic acid(RA) and telomerase antisense oligodeoxynucleotide (ASODN) respectively, the expression of CXCR4 mRNA and CXCR4 protein in NPC CNE1 and CNE2Z cells were determined by in situ hybridization and immunohistochemistry, respectively; the distribution of cell cycle was examined with flow cytometry and the proliferation of cells was identified by MTT method. RESULTS:CXCR4 mRNA and CXCR4 protein were strongly expressed in both CNE1 and CNE2Z cells, and their expression in CNE2Z cells was stronger than that in CNE1 cells. After treated with 1×10-5 mol/L and 1×10-4 mol/L RA, CNE1 cells were arrested in G1 phase and CNE2Z cells in S phase, while the CXCR4 mRNA expression was significantly decreased in both CNE1 and CNE2Z cells compared with control group cells (P< 0.01).The effect of 1×10-4 mol/L RA was more powerful than that of 1×10-5 mol/L RA. After treated with ASODN, the proliferation of CNE1 and CNE2Z cells was inhibited, and the expression of CXCR4 protein was decreased compared with the control (P< 0.01). CONCLUSION:CXCR4 is highly expressed in NPC cells,and its expression was associated with differentiation grade and proliferation ability of NPC cells.

关 键 词：鼻咽癌 趋化因子受体 CXCR4 SDF-1 全反式维甲酸 端粒酶反义核酸 

分 类 号：R739.63[医药卫生—肿瘤]