卵巢上皮癌组织中survivin的表达及其与Fas、FasL表达的关系  

Expression of Survivin in Ovarian Epithelial Carcinoma and Its Correlation with Expression of Fas and FasL

作　　者：马湘一[1] 何福仙[1] 邬素芳[1] 卢运萍[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《癌症》2004年第2期173-176,共4页Chinese Journal of Cancer

基　　金：国家杰出青年基金资助项目(No.30025017);国家重点基础研究发展项目(No.2002CB513100)~~

摘　　要：背景与目的:近期研究表明,凋亡抑制蛋白survivin在细胞凋亡和有丝分裂中起重要作用,于多种常见的恶性肿瘤有表达。本研究拟探讨survivin在卵巢上皮癌中的表达,及其与Fas、FasL表达的相互关系。方法:用免疫组织化学SP法检测84例卵巢上皮癌组织、39例卵巢良性肿瘤组织和20例正常卵巢组织中survivin、Fas、FasL的表达。结果:63.1%的卵巢上皮癌组织表达survivin基因,良性肿瘤组织表达率为30.8%,而正常卵巢组织中无表达,差异有显著性(P<0.01)。Survivin表达的高低与临床分期、组织学分级等密切相关。Fas在卵巢上皮癌组织中的表达(23.8%)显著低于卵巢良性肿瘤组织(53.8%)(P<0.01);而FasL在卵巢上皮癌组织中的表达(44.0%)则显著高于卵巢良性肿瘤组织(23.1%)(P<0.05)。survivin的表达与Fas、FasL的表达密切相关。结论:(1)Survivin基因在卵巢上皮癌组织中表达上调;(2)Fas与FasL在卵巢上皮癌中的异常表达及其与survivin基因的表达密切相关,说明它们在卵巢上皮癌的发生发展过程中可能通过共同的机制参与卵巢上皮癌的发生。BACKGROUND &OBJECTIVE: Recent research had shown that survivin, a new member of the inhibitors of apoptosis proteins (IAP) family, which also plays an important role in mitosis and cell apoptosis, and selectively overexpressed in common human cancers. This study was designed to examine the expression of survivin and its correlation with expression of Fas and FasL in ovarian epithelial carcinoma. METHODS: Immunohistochemical assay (SP method) was used to detect the expression of survivin, Fas and FasL genes in 84 ovarian cancer tissues, 39 benign tumors of ovary, and 20 normal ovary tissues. RESULTS: The level of expression of survivin was higher in the patients with ovarian cancer (63.1%) than that in the patients with benign tumor of ovary (30.8%) and normal ovary tissues (0.0%)(P< 0.01). The expression level of survivin was strongly correlated with clinical stage and poor differentiation. The expression of Fas in ovarian cancer (23.8%) was significantly lower than that of ovarian benign tumor (53.8%) (P< 0.01). The expression rate of FasL was significantly higher in ovarian cancer (44.0%) than that in ovarian benign tumor (23.1%)(P< 0.05). Positive survivin expression was strongly correlated with Fas and FasL expression. CONCLUSION: (1) High expression of survivin may plays an important role in the development of ovarian cancer and could be a useful prognostic maker for patients with ovarian cancer.(2) The abnormal expression of Fas and FasL in ovary cancer and their correlation with survivin suggested that survivin might be in cooperation with Fas and FasL,which involved in the pathogenesis of ovarian cancer.

关 键 词：卵巢上皮癌 SURVIVIN FAS FASL 免疫组织化学 发病机制 

分 类 号：R737.31[医药卫生—肿瘤]