激光激活BPD-MA光动力作用对人膀胱癌细胞株BIU-87DNA合成的影响  

Effect of photodynamic therapy with laser-activated BPD-MA on DNA synthesis of human bladder cancer BIU-87 cells

作　　者：许川山[1] 余茜[2] 吴士明[3] 唐建民[3] 石含英[1] 杨青[1] 肖斌[1] 

机构地区：[1]第三军医大学西南医院整形康复中心激光研究室,重庆400038 [2]四川省泸洲医学院附属医院康复科,泸洲646000 [3]第三军医大学新桥医院激光医学研究中心,重庆400037

出　　处：《激光杂志》2004年第1期84-86,共3页Laser Journal

摘　　要：目的 :研究光动力抑癌作用机制。方法 :采用3H -TdR掺入法检测激光光敏化BPD -MA后人膀胱癌细胞株BIU -87细胞DNA的合成。结果 :实验前对照组的CPM值为 482 3 .5± 5 4.2 6,激光激活BPD -MA光动力显著抑制人膀胱癌细胞株BIU -87细胞DNA的合成 (12 63 .7± 2 0 6.84VS 482 3 .5± 5 4.2 6,P <0 .0 5 )。而单纯光敏剂BPD -MA和假照射处理对人膀胱癌细胞株BIU -87细胞DNA合成无明显影响 (P >0 .0 5 )。结论 :激光激活BPD -MA光动力抑制人膀胱癌细胞株BIU -87细胞DNA的合成可能是其抑癌作用机制之一。Objectives:To study on the mechanisms of photodynamic therapy on malignant tumors.Method:Photosensitization of BPD-MA was activated by laser with red light(632.8nm)delivered at 10 mW/cm 2 to give a total dose of 2.4J/cm 2.DNA synthesis of human bladder cancer BIU-87 cells was determined with 3 H-TdR incorporation.Results:A baseline proliferative activity in BIU-87 cells before laser irradiation without BPD-MA was maintained at a rate of 3 H-thymidine incorporation of 4823.5±54.26CPM.Treatment of cells with photodynamic therapy significantly decreased cellular proliferative activity as measured by 3 H-thymidine incorporation (1263.7±206.84 VS 4823.5±54.26, P<0.05).However,Laser irradiation alone,BPD-MA alone and sham radiation without BPD-MA did not affect the DNA synthesis of human bladder cancer BIU-87 cells (P>0.05).Conclusion:Treatment of cells with photodynamic therapy significantly decreases cellular proliferative activity as measured by 3 H-thymidine incorporation,which is one of the mechanisms of photodynamic therapy on malignant tumors.

关 键 词：光动力治疗 膀胱癌 细胞株 BIU-87细胞 DNA合成 生物学机制 激光激活BPD-MA光动力 

分 类 号：R737.14[医药卫生—肿瘤] R730.57[医药卫生—临床医学]