烟叶中β胡萝卜素的分离纯化和测定  

Isolation of β-Carotene from Discarded Tobacco Leaves

作　　者：夏薇[1] 陈新志[1] 

机构地区：[1]浙江大学材料与化工学院,浙江杭州310027

出　　处：《色谱》2004年第1期54-56,共3页Chinese Journal of Chromatography

摘　　要：采用直接柱色谱的方法,用石油醚单一溶剂作为洗脱液,从霉烟或碎烟末浸膏中提取了β 胡萝卜素纯品。通过1H NMR和13C NMR谱图鉴定了β 胡萝卜素的结构。用高效液相色谱法(HPLC)测得β 胡萝卜素纯品的纯度为98%,测定方法准确性高、重现性好,β 胡萝卜素纯品的红外光谱图和熔点均与文献值相符。利用该方法提取β 胡萝卜素纯品,不仅收率达到80%,纯度好,而且单一溶剂作为洗脱液很容易回收再利用,因此说该方法是一种有价值的生产天然β 胡萝卜素的方法,具有良好的工业应用前景。β-carotene was isolated from discarded tobacco leaves by column chromatography using petroleum ether as eluent. Its structure was characterized by ~1H-NMR and ~13C-NMR spectra. The purity of theβ-carotene was determined to be 98% by a high performance liquid chromatographic (HPLC) method which was demonstrated to be accurate and reproducible. Infrared spectrum and melting point of the isolated sample were also characterized in agreement with those reported in literature. The overall yield of 80%, the high purity and the easiness to recycle as the results of only a single solvent employed, would make the method an important reference for future research of β-carotene and a favorable technique for future development.

关 键 词：烟叶 Β-胡萝卜素 分离 纯化 测定 结构鉴定 石油醚 直接柱色谱 废物利用 

分 类 号：TS49[农业科学—烟草工业] Q562[生物学—生物化学]