电穿孔化疗对结肠癌裸鼠移植瘤模型的作用研究  

Effects of electrochemotherapy on human colon cancer xenografts in nude mice

作　　者：郑民华[1] 冯波[1] 孙骥远[1] 陆爱国[1] 李健文[1] 王明亮[1] 郁宝铭[1] 

机构地区：[1]上海第二医科大学附属瑞金医院外科,上海市微创外科临床医学中心,上海消化外科研究所,上海200025

出　　处：《外科理论与实践》2004年第1期50-53,共4页Journal of Surgery Concepts & Practice

基　　金：上海市卫生局基金资助项目(00440)

摘　　要：目的:观察电穿孔技术结合博莱霉素化疗对结肠癌裸鼠移植瘤的抗肿瘤效果,为本疗法的临床应用提供实验依据。方法:于裸鼠左前肢皮下接种人结肠癌LoVo单细胞悬液,3周后成功建立结肠癌裸鼠皮下移植瘤模型40只,随机将其分为电穿孔化疗(B+E+)组、单纯化疗(B+E-)组、单纯电穿孔(B-E+)组和阴性对照(B-E-)组,每组10只。动态观察肿瘤大小,治疗第7天处死小鼠并取出皮下肿瘤称重,。并观察其组织学改变。结果:B+E+组裸鼠移植瘤体积较其他各组明显缩小(P<0.01),该组1只裸鼠的移植瘤呈完全缓解,部分缓解7只;B-E+和B+E-组内各有1只部分缓解,而B-E-组中无一有效。B+E+组有效率与其余各组的差别有统计学意义(P<0.01)。组织学观察也显示B+E+组的移植瘤内大片肿瘤细胞坏死、血管损伤和炎细胞浸润。结论:电穿孔化疗可显著增强结肠癌裸鼠皮下移植瘤对化疗的敏感性,疗效明显,有可能成为结直肠癌治疗的一种新方法。Objective To observe the combined anti-tumor effect of electroporation and bleomycin (BLM) in human colon cancer xenografts in nude mice, and to establish the experimental basis for clinical application of electrochemotherapy(ECT). Methods Forty nude mice,inoculated subcutaneously with human colon cancer cell line LoVo for three weeks, were allocated randomly into 4 groups: B+E+(electrochemotherapy), B+E-(intratumoral bleomycin injection only), B-E+(electrotherapy only), and B-E-(no treatment). Tumor volumes were measured daily. The animals were sacrificed on the seventh day after the treatments, the xenografts were weighed, and studied histologically. Results The mean tumor volume of Group B+E+ animals was statistically lower than the other 3 groups ( P<0.01). There were 1 animal of complete response(CR) and 7 animals of partial response(PR) in Group B+E+; 1 animal of PR in Group B+E- and Group B-E+ respectively, and no response was observed in group B-E-. The difference in response observed between Group B+E+ and the other three groups was statistically significant (P<0.01). Histologically, extensive necrosis of the tumor cells with considerable vascular damage and inflammatory cells infiltration were observed in Group B+E+. Conclusions Electrochemotherapy could significantly enhance the chemosensitivit of human colon cancer xenografts in nude mice, and could serve as new mode treatment for human colon cancer.

关 键 词：电穿孔化疗 结肠癌 裸鼠 移植瘤 动物模型 博莱霉素 

分 类 号：R735.35[医药卫生—肿瘤] R73-361[医药卫生—临床医学]