缺氧对人视网膜色素上皮细胞增生和VEGF表达的影响  

Influence of hypoxia on proliferation of cultrued human retinal pigment epithelial cells and expression of VEGF

作　　者：张鹏[1] 王雨生[1] 张星[2] 惠延年[1] 胡丹[1] 马吉献[1] 

机构地区：[1]第四军医大学西京医院眼科 [2]第四军医大学基础部

出　　处：《眼科新进展》2004年第1期6-8,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助 (编号 :30 371 51 6);第四军医大学科技创新工程基金资助(编号:CX0 2A0 2 1 )~~

摘　　要：目的　观察缺氧对培养的人视网膜色素上皮 (retinalpigmentepithelial,RPE)细胞增生和血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)表达的影响。 方法将培养的人RPE细胞接种于 2 4孔板 ,分别放入正常和缺氧环境中培养 ,于 1、2、3、4、5d后用四甲基偶氮唑蓝 [3 ( 4 ,5 dimethylthiazole 2yl) 2 ,5 diphenyltetrazoliumbromide ,MTT]比色法检测RPE细胞的增生 ,于 6、12、2 4h后用免疫组织化学法检测RPE细胞对VEGF的表达 ,经计算机图像处理 ,定量分析。结果　缺氧组的MTT实验吸光度 (A值 )均高于正常组 (P <0 .0 1) ;抗VEGF抗体染色 ,缺氧组明显强于正常组 (P <0 .0 5 )。结论缺氧可促进RPE细胞的增生及其对VEGF的表达 。Objective To study the influence of hypoxia on proliferation of cultured human retinal pigment epithelial(RPE)cells and expression of VEGF.Methods The human RPE cells were passaged into 24 well dishes,and the dishes were put into normal and hypoxic chamber respectively.After 1day,2,3,4 and 5 days,the proliferation of RPE cells was evaluated by [3 (4,5 dimethylthiazole 2yl) 2,5 diphenyl tetrazolium bromid,MTT] test.After 6,12,24 hours,the VEGF protein of each group was determined with immunohistochemical assay;image processing and quantitative analysis were applied with computer.Results The A value in the hypoxia group was higher than that in the normal group at all time points( P < 0.01 ),the expression of VEGF in human RPE cells increased under hypoxia( P < 0.05 ).Conclusion Hypoxia can stimulate the proliferation of human RPE cells and increase the expression of VEGF in RPE cells.The results indicate that controlling the activity of RPE during hypoxia is essential to inhibit the formation of choroidal neovascularization.

关 键 词：缺氧 视网膜色素上皮细胞 血管内皮生长因子 细胞培养 细胞增生 

分 类 号：R774[医药卫生—眼科]