新生儿口腔中白色念珠菌来源的分子遗传学初探  

A molecular and genetical study of the source of Candida albicans in the mouth of neonates

作　　者：曾昕[1] 陈谦明[1] 李晴 刘植华 聂敏海[3] 李秉琦[1] 

机构地区：[1]四川大学华西口腔医学院,成都610041 [2]深圳市妇女儿童医院 [3]泸州医学院

出　　处：《实用口腔医学杂志》2004年第1期108-110,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金资助课题课题编号:30 0 70 81 4

摘　　要：目的 :比较新生儿口腔中与其母亲口腔和阴道白色念珠菌基因型的差异 ,探讨新生儿口腔中白色念珠菌的来源。方法 :采集 2 0 8例产妇产前 48h以内的口腔含漱液标本和阴道拭子标本及其足月新生儿 1、3、5d和 1月的口腔拭子标本 ,进行白色念珠菌的培养和鉴定。再选取母子均分离出白色念珠菌的标本进行DNA提取和随机引物扩增DNA多态性分析法 (RAPD)分析 ,比较基因型是否具有差异。结果 :产后 5d内仅有不到 1%的新生儿口腔中检出了念珠菌 ,产后 1月新生儿口腔中白色念珠菌的检出率增高为 2 1.3 9%。并且 ,从新生儿分离得到的白色念珠菌的基因型与从其母亲口腔或阴道分离得到的白色念珠菌的基因型均不相同。结论Objective: To investigate the source of Candida alb ic ans in the mouth of neonates. Methods: 208 mother in fant pairs were recruited. Specimens from mothers for fungal culture were obtain de from the mouths by oral rinse and from vagina by swab within 48 h before de livery, and specimens from full term neonates were obtained from mouth by swab on the 1st, 3rd and 5th day while still in hospital and when the neonates were 1 month old. Candida species were identified by the routine microbiologica l methods, then Candida albicans isolated from mother infant pairs were com pared genetically by random amplified polymorphic DNA analysis (RAPD). Results: Candida albicans was isolated in the mouth of only 2 neonates (0.96%) and in the vagina and mouth of the corresponding 2 mothers in hospital.That was isola ted in the mouth of 43 neonates (21.39%) when they were 1 month old and in both vagina and mouth of 3 corresponding mothers and in the mouth of another 2 corr esponding mothers. RAPD revealed different genotypes of Candida albicans in each mother infant pair. Conclusions: The vertical tran smission of Candida albicans from mother to baby seems not to be the main so urce of Candida albicans in neonate mouth.

关 键 词：新生儿 白色念珠菌 分子遗传学 基因型 口腔念珠菌病 

分 类 号：R788[医药卫生—口腔医学]