江苏省2012-2013年柯萨奇病毒A组6型分子流行特征分析  

Genetic characteristics of coxsakievirus A6 circulated in Jiangsu province of China from 2012 to 2013

作　　者：卞莲莲[1] 姚昕[1] 毛群颖[1] 叶强[1] 朱凤才[2] 梁争论[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]江苏省疾病预防控制中心,南京210000

出　　处：《中国病毒病杂志》2015年第5期361-367,共7页Chinese Journal of Viral Diseases

基　　金：国家"十二五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2013ZX09304101)

摘　　要：目的分析江苏省2012-2013年流行的柯萨奇病毒A组6型(coxsackievirus A6,CV-A6)毒株VP1区序列特征。方法采集江苏省2012-2013年10例CV-A6感染引发的手足口病(hand,foot and mouth disease,HFMD)患儿的咽拭子或肛拭子样本,通过RT-PCR扩增完整VP1区。应用DNAStar软件包的MegAlign对CV-A6毒株的VP1区进行核苷酸和氨基酸序列比对分析,构建系统进化树。结果同源性和系统进化分析结果显示,10株流行株与2008年芬兰流行株同属于分支F,核苷酸同源性为95.1%~96.5%,而与原型株Gdula核苷酸同源性较低,为82.4%~84.0%。01-PZ/JS/2012、02-PZ/JS/2012、03-PZ/JS/2012、04-BY/JS/2012和08-PZ/JS/2013属于分支F2,05-PZ/JS/2013、06-PZ/JS/2013、07-PZ/JS/2013、09-PZ/JS/2013和10-BY/JS/2013属于F3,不同亚分支毒株之间核苷酸同源性为95.8%~97.2%。结论江苏省2012-2103年流行的10株毒株分别属于F2和F3分支,属于F2的5株毒株的VP1第30位氨基酸位点为V,而属于F3的5株毒株该位点为A。开展CV-A6流行株分子流行病学研究,了解毒株进化变异的趋势,对防控CV-A6引发的HFMD具有重要意义。Objective To study the VP1 region of coxsakievirus A6(CV-A6)strains circulated in Jiangsu province of China. Methods Throat swab and stool swab samples were collected from HFMD(hand,foot and mouth disease)patients infected with coxsackievirus A6(CV-A6)in Jiangsu province of China from 2012to2013.The VP1 region sequences were amplified by RT-PCR and the nucleotide identity between sequences was calculated and sequence alignments were made to generate phylogenetic trees using MegAlign in DNAStar.Results Nucleotide identity and phylogenetic analysis based on the VP1 region sequences showed that the 10 srains belonged to lineage F and share 95.1%-96.5% nucleotide homology with the Finland strain of 2008 but relatively lower nucleotide homology(82.4%-84.0%)with the prototype strain(Gdula).Strains 01-PZ/JS/2012,02-PZ/JS/2012,03-PZ/JS/2012,04-BY/JS/2012 and 08-PZ/JS/2013 were grouped to sub-lineage F2 while 05-PZ/JS/2013,06-PZ/JS/2013,07-PZ/JS/2013,09-PZ/JS/2013 and 10-BY/JS/2013 were categorized to F3,and the nucleotide homology were 95.8%-97.2%.The 30 th amino acid of the five strains in F2 was V,while the five in F3 was A. Conclusions The CV-A6 strains circulated in Jiangsu province of China in 2012 and 2013were mainly F2 and F3,respectively.

关 键 词：手足口病(HFMD) 柯萨奇病毒A组6型(CV-A6) 基因型 

分 类 号：R725.1[医药卫生—儿科]