p63基因在人类肺癌组织中转录表达水平的研究  

The expression of p63 gene in the lung cancer of human

作　　者：郭伟[1] 范士志[1] 蒋耀光[1] 李志平[1] 陈建明[1] 牛会军[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所全军胸心外科中心,重庆400042

出　　处：《重庆医学》2004年第2期182-184,共3页Chongqing medicine

摘　　要：目的　研究p6 3基因在人类非小细胞肺癌 (NSCLC)、小细胞肺癌 (SCLC)组织中转录表达水平及其意义。方法　采用RT PCR(reversetranscriptase PCR)方法对 4 0例非小细胞肺癌 ,6例小细胞肺癌 ,同时对 1 0例良性疾病肺组织中p6 3基因两种亚型 (TAp6 3及ΔNp6 3)转录表达情况进行检测和比较 ,同时用免疫组化检测P6 3蛋白在上述组织中的表达情况。 结果　 1 9例NSCLC肿瘤组织 (1 9/40 ) ,5例SCLC肿瘤组织 (5 /6 )及 2例癌旁组织 (2 /46 )中ΔNp6 3呈阳性表达 ;良性疾病肺组织中TAp6 3与ΔNp6 3表达均为阴性。P6 3蛋白在肺鳞状细胞癌组织及小细胞肺癌组织中的阳性表达率和表达强度明显大于腺癌组织及良性病变肺组织 (P <0 .0 1 )。结论　p6 3基因在肺鳞状细胞癌及SCLC组织中呈阳性表达 ,其中主要是ΔNp6 3表达水平升高 ,而P6 3蛋白在肺鳞状细胞癌及SCLC组织中表达水平明显高于腺癌组织 ,推测ΔNp6 3基因在肺鳞状细胞癌及SCLC组织中转录表达水平升高可能抑制细胞凋亡 ,促进肿瘤增殖 。Objective To investigate the significance of the expression of p63 gene in the non small sell lung carcinoma(NSCLC),and small cell lung carcinoma(SCLC).Methods RT PCR(reverse transcriptase PCR) technique was used to observe p63 gene′s expression(TAp63 and ΔNp63) in 40 cases of NSCLCs,6 SCLCs and 10 benign disease tissues of lung,and imunohistochemical staining to was used observe p63 protein′s expression in these tissures.Results ΔNp63 overexpressed in 19 cases of NSCLCs(19/40),5 SCLCs(5/6) and 2 paracarcinoma tissues(2/46).There was no expression of TAp63 in NSCLC,SCLC and lung benign disease tissues.The posive staining rates and intensity of p63 protein in squamal cell carcinoma and SCLC were significantly higher than those in lung adenocarcinoma and benign disease tissues of lung( P <0.01).Conculusion p63 gene is amplified in squamal cell carcinoma and SCLC.This maybe due to the function of p63 in forming and development of squamal cell carcinoma and SCLC.The overexpression of ΔNp63 in squamal cell carcinoma and SCLC can suppress the apoptosis and accelerate the prolifetation of tumour,so maybe ΔNp63 is an oncogene amplified in NSCLCs and SCLCs.

关 键 词：P63 非小细胞肺癌 小细胞肺癌 P53基因家族 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]