福尔奔胶囊对人成骨肉瘤细胞HOS TE85增殖及Ⅰ型胶原产生的影响  

Effects of Fullbone Capsules on the Proliferation of, and the Type I Collagen Synthesis in Human Osteoblastic Sarcoma Cell TE85

作　　者：龚海洋[1] 李文仙[2] 金莉[2] 孙兰[3] 刘景生[3] 

机构地区：[1]北京中医药大学,北京100029 [2]中国疾病预防控制中心,北京100050 [3]中国协和医科大学,北京100005

出　　处：《北京中医药大学学报》2004年第1期24-26,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目 (No .3 943 0 12 0 )

摘　　要：目的　通过体外培养的人成骨肉瘤细胞HOSTE85 ,观察福尔奔胶囊 (Fullbone)对成骨细胞增殖及Ⅰ型胶原产生的影响。方法　在体外培养的人成骨肉瘤细胞HOSTE85培养液中 ,加入Full bone (1～ 10 0 0 0 μg/L) ,分别培养 2 4、4 8、72、96h ,于结束培养前 6h ,加入3H -脱氧胸腺嘧啶检测细胞增殖 ;或于细胞开始培养时 ,加入3H -脯氨酸检测Ⅰ型胶原产生。结果　在 4 8、72h ,Fullbone明显抑制细胞增殖 ,量效关系显著 ;在 4 8、72、96h ,Fullbone明显刺激细胞Ⅰ型胶原产生 ,量效关系显著。结论　Fullbone对人成骨肉瘤细胞HOSTE85增殖具有抑制作用 ,对Ⅰ型胶原产生具有促进作用 ,这可能是其临床治疗更年期骨质疏松的作用机理。Objective To observe the effects of Fullbone Capsules (FC) on the proliferation of cultured human osteobalstic sarcoma cell TE85 (HOS TE85) and on type I collagen synthesis (T I CS) in HOS TE85. Methods FC (1 10000 ug/L) was added into the HOS TE85 culture fluid and was cultured 24 hours, 48 hours, 72 hours and 96 hours, respectively. 3H thymidine was added into the HOS TE85 culture fluid 6 hours before finishing the culture to detect the proliferation of HOS TE85; 3H proline was added into the HOS TE85 culture fluid at the beginning of the culture to detect T I CS. Results FC could remarkably restrain the proliferation of HOS TE85 at the 48th hour and the 72nd hour, and markedly promote T I CS at the 48th hour, the 72nd hour and the 96th hour, with an obvious dose effect relationship.Conclusion FC exert effects of restraining the proliferation of HOS TE85 and promoting the T I CS, which may be FC's mechanism of treating postmenopausal osteoporosis.

关 键 词：福尔奔胶囊 成骨细胞 增殖 Ⅰ型胶原 

分 类 号：R285.5[医药卫生—中药学]