肌形成蛋白在人体失神经骨骼肌表达的形态学  

Morphological Observation of Myogenin Expression in Denervated Human Skeletal Muscles

作　　者：黄汉伟[1] 徐建光[2] 顾玉东[2] 

机构地区：[1]福建医科大学附属第一医院骨科,福州350005 [2]复旦大学上海华山医院手外科,上海20004

出　　处：《福建医科大学学报》2004年第1期18-20,F002,共4页Journal of Fujian Medical University

摘　　要：目的　研究肌形成蛋白在人体失神经骨骼肌中的表达规律。　方法　成人骨骼肌 1 8例 ,正常骨骼肌 3例 ,失神经时间 1 2个月内骨骼肌 9例 ,1 3～ 2 4个月 6例。免疫组织化学染色方法 (ABC法 )研究肌形成蛋白在骨骼肌内的表达。　结果　正常骨骼肌未见肌形成蛋白表达 ,失神经人体骨骼肌内肌形成蛋白蛋白表达在肌纤维细胞核与肌卫星细胞细胞核 ;失神经 1年内可见到肌形成蛋白阳性染色细胞核 ,之后阳性肌细胞核明显减少 ,具有统计学意义 (P<0 .0 5 )。　结论　人体骨骼肌失神经 1年后再生反应明显降低 ,提示人体骨骼肌失神经后应该尽早修复。Objective To investigate the expression of myogenin in denervated human skeletal muscles. Methods 18 cases of human skeletal muscles, healthy skeletal muscles 3 cases, 1~12 month denervated skeletal muscles 9 cases and 13~24 month denervated skeletal muscles 6 cases were recruited. Immunohistochemical method(ABC) and HE staining were used to study the expression of myogenin. Results Myogenin expression were located in the nuclei of muscle fibers and nuclei of satellite cells in denervated skeletal muscles, but not in normal cases. Some myogenin positive neclei were found in the group with denervation period less than one year, and then its number decreased significantly(P<0 05). Conclusion Human skeletal muscles with nerve injury less than one year denervation is beneficial to reinnervation, so human peripheral nerve should be repaired as soon as possible after injury.

关 键 词：肌形成蛋白 肌 骨骼 周围神经 

分 类 号：R361[医药卫生—病理学]