复方参七汤在人结肠癌细胞株裸鼠肝转移中的应用  

Experimental study of the effect of compound shenqitang on nude mice with liver metastases

作　　者：陆飞[1] 蔡兵[1] 沈洪薰[2] 

机构地区：[1]江苏省无锡市第一人民医院肝胆外科,无锡214000 [2]南通医学院附属医院普外科

出　　处：《南通医学院学报》2004年第1期47-49,52,共4页ACTA Academiae Medicinae Nantong

摘　　要：目的 :建立人结肠癌细胞株裸鼠肝转移模型 ,研究复方参七汤对裸鼠肝转移的影响。方法 :对 2 4只BAL B/ C裸鼠 ,经脾脏注入指数生长期的人结肠癌 (低分化腺癌 )细胞悬液 (L s174 t) 0 .1ml,含细胞数 1× 10 6个 ,切除脾脏 ,分成实验组和对照组各 12只。实验组每天喂以含 0 .6 ml/ 2 0 g体重复方参七汤颗粒饲料 ,对照组裸鼠喂以颗粒饲料 ,记录裸鼠体重、神志、摄食。喂养 3周后 ,抽取各组裸鼠心脏血后处死 ,观察腹腔内肿瘤生长情况 ,检查肝转移癌灶数目 ,挖出各个癌结节并称重。标本作病理组织学检查 ,同时采用免疫组化 (SP)方法检测肿瘤转移抑制基因 nm2 3H1- NDPK表达 ;采用巢式逆转录聚合酶链反应 (RT- PCR)法 ,检测裸鼠血中 CK2 0 m RNA表达。结果 :(1)建立人结肠癌细胞株裸鼠肝转移模型 ,肝转移率 10 0 %。病理组织学证实肝转移癌细胞具有人结肠癌 (低分化腺癌 )特征。 (2 )实验组裸鼠实验前后体重增长 4 .12± 0 .5 3g,明显高于对照组 3.2 1± 0 .5 9g(P<0 .0 5 )。 (3)实验组裸鼠肝转移癌结节的数目 2 .4 2± 0 .99个 ,明显低于对照组 4 .17± 1.99个 (P<0 .0 5 )。(4 )实验组裸鼠肝转移癌重 6 3.6 7± 2 1.2 9m g,明显低于对照组 94 .32± 37.86 m g(P<0 .0 5 )。 (5 )实验组裸鼠肝转移癌重Objective:To establish a colonic adenocarcinoma liver metastases model for experimental study of the effect of compound shenqitang.Methods:Liver metastases were established by intrasplenic injection of colonic adenocarcinoma cells(Ls174t) with 1×10 6 cells in 0.1ml solution in 24 BALB/c nude mice which were divided into two groups randomly.Granular forage with compound shenqitang was fed in experimental group and granular forage without compound shenqitang was fed in control group daily. After three weeks every mouse was weighted and killed,blood of the mice should be collected .The weight,size and the number of the liver metastatic lesions were studied,every carcinoma node should be weighted,specimens were fixed by formalin and embedded by paraffin ,all slices with a thickness of 4μm were studied histologically.nm23H 1-NDPK expression in 24 nude mice with liver metastases from colon carcinoma was examined immunohistochemical (sp) by using antinm23.Using nested reverse transcript polymerase chain reaction(RT-PCR) technique CK20 mRNA in blood of 24 nude mice with liver metastasis from colon carcinoma was detected.Results:All mice inoculated with Ls174t cell lines developed liver metastases,other organic metastases weren't observed.The pathological results showed that tumour cells of liver metastases accorded with poorly differentiated human colonic adenocarcinoma . In experimental group the growth weight(4.12±0.53g) from experimental opening to ending was significantly higher than that(3.21±0.59g) in control group(P<0.05). In experimental group the number(2.42±0.99n) and the weight(63.67±21.29mg) of liver metastases were significantly lower than those(4.17±1.99n,94.32±37.86mg) in control group(P<0.05). In experimental group nm23H 1-NDPK positive expression rate (66.7%) significantly higher than that(16.7%) in control group(P<0.05). Using semiquantitative examination, In experimental group the relative value of CK20 mRNA expression in blood (0.398±0.143) was significantly lower than that(0.518±0.09

关 键 词：结肠癌 肝转移 裸鼠模型 nm23-H1 CK20 mRNA 巢式逆转录聚合酶链反应 

分 类 号：R735.35[医药卫生—肿瘤]