豆类重要植物病原细菌检测研究进展  

Advances in detection of plant pathogenic bacteria in Leguminosae

作　　者：李志锋[1] 王忠文[1] 冯建军[2,3] 吴绍精 娄定风 王红英[2,3] 章桂明 

机构地区：[1]广西大学农学院,广西南宁530004 [2]深圳市检验检疫科学研究院深圳市外来有害生物检测技术研发重点实验室 [3]深圳出入境检验检疫局动植物检验检疫技术中心

出　　处：《植物检疫》2015年第6期7-15,共9页Plant Quarantine

基　　金：深圳市海外高层次人才创新创业专项资金项目(KQC201109050077A);国家质检总局科技项目(2010IK257);深圳出入境检验检疫科研项目(SZ2011002)

摘　　要：随着全球经济一体化的推进,豆类种子及其他植物产品的国际贸易越来越广泛,尤其是我国进口大豆量的迅猛增加,有害生物的跨境传播风险也随之增大。本文简要介绍了豆类上6种重要植物细菌病害及其危害特征,综述了分离培养技术、生理生化技术等传统检测方法和血清学技术以及普通PCR、实时荧光PCR等现代分子生物学检测技术在豆类重要植物病原细菌检测上的应用,并对其优缺点加以分析。在此基础上,对高通量检测方法的应用前景进行展望。In recent years, international trade for Leguminosae seeds and other plant products were widespread with development of global integration, especially China's rapid increase of amount of soybean imports, which lead to increase the risk of cross-border spread of the pest. This paper briefly introduces six important plant pathogenic bacterial diseases in Leguminosae and their threat, also summarizes the progresses of the applications of the detection methods in Leguminosae, such as cultivation technology, serology, classical PCR and real-time fluorescent PCR, and so on, and furthermore analyzes the advantages and disadvantages of their methods. This article further prospects application foreground of high-throughput detection techniques.

关 键 词：植物病原细菌 豆科作物 检测 

分 类 号：S432.42[农业科学—植物病理学]