蒙古山羊和哈萨克山羊GOL A-DRB3基因的HaeⅢ酶切多态性分析  

Polymorphism Analysis of the GOLA-DRB3 Gene Digested with HaeⅢ in Mongolian Goat and Kazakh Goat

作　　者：孙东晓[1] 张沅[1] 李宁[2] 

机构地区：[1]中国农业大学动物科技学院,北京100094 [2]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《遗传》2004年第1期55-58,共4页Hereditas（Beijing)

基　　金：国家自然科学基金(30170676);国家基础研究重点发展规划(TG2000016105)项目资助~~

摘　　要：采用限制性内切核酸酶HaeⅢ对蒙古山羊和哈萨克山羊GOLA DRB3基因外显子2的285bp扩增产物进行了PCR RFLP多态性分析,共检测到17种基因型,由A、B、C、D、E、F和H等7个复等位基因控制;通过酶切图谱分析发现蒙古山羊和哈萨克山羊的GOLA DRB3基因外显子2的154、168和220位碱基表现出多态性。并对基因型频率和等位基因频率进行了统计分析,结果表明,GOLA DRB3基因的部分基因型频率和等位基因频率在两个群体之间差异显著(P<0.10或P<0.05)或极显著(P<0.01);χ2适合性检验结果表明,蒙古山羊和哈萨克山羊的GOLA DRB3基因外显子2的HaeⅢ酶切位点均未达到Hardy Weinberg平衡状态(P<0.01)。The exon2 of GOLA-DRB3 gene was amplified and a uniform fragment of 285bp was obtained in Mongolian Goat and Kazakh Goat.The 285bp PCR product was digested with restriction endomuclease HaeⅢ and genetic polymorphism was investigated by PCR-RFLP.Seventeen kinds of genotypes were found in two populations,which were controlled by seven alleles.There are significant differences in some genotypic frequencies and gene frequencies between the two populations(P<0.10,P<0.05,P<0.01);The results of χ~2 test showed that genotypes of GOLA-DRB3 gene in two populations did not fit with Hardy-Weinberg equilibrium(P<0.01).

关 键 词：蒙古山羊 哈萨克山羊 GOLA-DRB3基因 外显子 

分 类 号：S827[农业科学—畜牧学] Q953[农业科学—畜牧兽医]