蓝布正中总鞣质、游离没食子酸及鞣花酸的含量测定  

Determination of Total Tannins,Free Gallic Acid and Ellagic Acid from Geum Japonicum Thunb. var.Chinese F. bolle

作　　者：段灿灿 杨沙 刘方 陈衬心 张建永 DUAN Cancan;YANG Sha;LIU Fang;CHEN Chenxin;ZHANG Jianyong(Key Lab Basic Pharmacology of Ministry of Education and Joint International Research laboratory of Ethnomedicine of Ministry of Education,Zunyi Medical University,Zunyi 563000 Guizhou,China;School of Pharmacy,Zunyi Medical University,Zunyi 563000 Guizhou,China)

机构地区：[1]遵义医科大学基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室,贵州遵义563000 [2]遵义医科大学药学院,贵州遵义563000

出　　处：《中药新药与临床药理》2019年第8期965-969,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(81560736,81760746);贵州省科学基金项目(黔科合J字[2014]2182);贵州省教育厅科技拔尖人才支持项目(黔教合KY字[2017]078);贵州省国内一流学科建设项目(YLXKJS-YS-05)

摘　　要：目的建立蓝布正中总鞣质、没食子酸及鞣花酸含量的测定方法。方法以干酪素作为吸附剂,磷钼钨酸为氧化剂,没食子酸为对照品,在碱性条件下显色,建立紫外可见分光光度法测定蓝布正中总鞣质含量的方法;采用高效液相色谱(HPLC)法同时测定蓝布正中没食子酸和鞣花酸的含量,使用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为0.01%甲酸水溶液-甲醇,梯度洗脱,流速1.2 mL·min-1,柱温30℃,检测波长274 nm。结果以没食子酸计,总鞣质含量在1.018 0~10.180 0μg·mL-1范围内线性关系良好,平均回收率为99.82%,相对标准偏差(RSD)为1.75%。没食子酸和鞣花酸分别在2.405 0~54.113 0、5.275 0~118.690 0μg·mL-1呈良好线性关系,平均回收率为99.6%、99.3%,RSD分别为1.14%、0.88%。结论本研究所建方法操作简便、准确、重复性好,可更全面地反映蓝布正药材中的鞣质特征。Objective To establish a method for determining the contents of total tannins,gallic acid and ellagic acid in Geum Japonicum Thunb.var.Chinese F bolle.(Chinese name Lanbuzheng,LBZ).Methods The UV-vis spectrophotometry was used to determine the content of total tannins of LBZ with casein as sorbent,phosphorus molybdenum-tungsten acid as oxidant.Contents of gallic acid and ellagic acid were determined by HPLC.The separation was performed on the Eclipse XDB-C18 column(250 mm×4.6 mm,5μm)with gradient elution.The mobile phase was 0.01%formic acid water solution and methanol,and the flow rate was set as 1.2 mL·min-1.The column temperature was kept at 30℃.The detection wavelength was set at 274 nm.Results For determination of total tannins,the linear relationship of standard curve was good within the range of 1.018 0-10.180 0μg·mL-1,and the average recovery was 99.82%with RSD of 1.75%.The linear ranges of gallic acid and ellagic acid were within2.405 0-54.113 0,5.275 0-118.690 0μg·mL-1,respectively.The average recovery rates for gallic acid and ellagic acid were 99.6%and 99.3%with RSD of 1.14%and 0.88%.Conclusion These methods are simple,accurate and suitable for the determination of the total tannins,gallic acid and ellagic acid in LBZ.

关 键 词：蓝布正 没食子酸 鞣花酸 总鞣质 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]