毛竹笋蛋白的双向电泳分析  

Two-dimensional Electrophoresis Analysis of Protein in Moso Bamboo Shoots

作　　者：杨慧敏[1,2] 吴良如[1,2] 潘雁红 高贵宾[1,2] 钟浩 叶晓丹[1,2] 郑友苗 

机构地区：[1]国家林业局竹子研究开发中心,杭州310012 [2]浙江省竹子高效加工重点实验室,杭州310012

出　　处：《竹子研究汇刊》2015年第3期35-38,共4页Journal of Bamboo Research

摘　　要：以毛竹笋为材料,采用改良TCA-酚法提取竹笋总蛋白,Bradford法测定其浓度为9.470 mg·m L-1,采用1 500μg的上样量,使用24 cm,p H 3~10 NL IPG预制胶条进行总蛋白的双向电泳,凝胶图谱经PDquest 8.0软件分析,结果得出,毛竹笋的蛋白点数有1 014个,等电点主要集中在p H 6.0~7.0范围,相对分子质量集中在在45~116 k Da范围内,占58.48%。The total protein of moso bamboo shoots was extracted by the modified TCA-phenol method,and then determinated by Bradford method. The content of total protein was 9. 470 mg·m L- 1. Two-dimensional electrophoresis was carried out with 1500μg loading amount on 24 cm nonlinear IPG strips at p H 3- 10. Gel image was analyzed with PDquest 8. 0 software and the results showed that there were 1014 bamboo shoot protein points,isoelectric points mainly concentrated in p H 6. 0- 7. 0 and relative molecular mass concentrated in the range of 45- 116 k Da,accounting for 58. 48%.

关 键 词：毛竹 冬笋 蛋白 双向电泳 

分 类 号：S644.2[农业科学—蔬菜学]