The Role of β3-adrenergic Receptor Gene in Neuropeptide Y Y5Receptor Antisense Gene Therapy of Diet-induced Obese Rats  

β3肾上腺素能受体基因在肥胖大鼠神经肽Y Y5受体反义基因治疗中的作用(英文)

作　　者：张敏[1] 李晓南[1] 郭锡熔[1] 龚海霞[1] 丁胜利[1] 费莉[2] 刘倩琦[2] 陈荣华[2] 

机构地区：[1]南京医科大学第二附属医院儿保科,南京210011 [2]南京医科大学儿科医学研究所

出　　处：《Journal of Nanjing Medical University》2004年第1期16-20,共5页南京医科大学学报（英文版）

基　　金：SupportedbygrantfromNationalNaturalScienceFoundation ( 3 9870 3 62 )andNaturalScienceFoundationofEducationCommitteeofJiangsuProvince ( 98KJB3 2 0 0 0 2 )

摘　　要：Objective: To study the role of β3-adrenergic receptor gene in neuropeptide Y(NPY) Y5 receptor antisense gene therapy of diet-induced obese rats.Methods: The diet-induced obese rats were prepared by feeding a high-nutrition diet. Lateral ventricular was cannulated in obese rats which then received an intraventricular injection of either 5 μg/μl NPY Y5 receptor antisense or 10 μl missense oligodeoxynucleotide or saline of 10 μl respectively in every rat. When the rats were killed, the wet weight of abdominal adipose tissue, the level of serum lipid and lipoprotein were measured. Total RNA from the retroperitoneal adipose tissue was extracted and the level of β3-adrenergic receptor gene mRNA expression was evaluated by RT-PCR.Results: ①The wet weight of abdominal adipose tissue, the levels of serum lipids were greatly higher in diet-induced obese rats than those in normal rats. However, there were much lower β3-adrenergic receptor gene mRNA expression levels in retroperitoneal adipose tissue in diet-induced obese rats as compared with those in normal rats. ②After the diet-induced obese rats were intraventricularly administered with NPY Y5 receptor antisense oligodeoxynucleotide, the levels of β3-adrenergic receptor gene mRNA expression in retroperitoneal adipose tissue of diet-induced obese rats were strikingly up-regulated, whereas the wet weight of abdominal adipose tissue, the levels of serum lipids were markedly reduced.Conclusion: Intraventricular administration of antisense oligodeoxynucleotide to NPY Y5 receptor could significantly reduce the abdominal adipose tissue and the levels of serum lipids in diet-induced obese rats by up-regulating the level of β3-adrenergic receptor gene mRNA expression in retroperitoneal adipose tissue.目的 :探讨 β3肾上腺素能受体基因在肥胖大鼠神经肽Y(NPY)Y5受体反义基因治疗中的作用。方法 :建立高营养饮食诱导的肥胖大鼠模型 ,侧脑室插管后注射NPYY5受体反义、错义寡核苷酸或生理盐水 ,测定肥胖大鼠的腹腔内脂肪组织湿重与血脂 ,并采用RT PCR技术检测腹膜后脂肪组织中 β3肾上腺素能受体基因的表达水平。结果 :①高营养饮食诱导下肥胖模型大鼠的腹腔脂肪组织湿重与血脂水平均明显高于正常大鼠 ;②经NPYY5受体反义寡核苷酸干预后 ,肥胖大鼠的腹腔脂肪组织湿重与血脂水平均明显降低 ,腹膜后脂肪组织中 β3肾上腺素能受体基因的表达水平则显著高于肥胖对照大鼠。结论 :侧脑室注射NPYY5受体反义寡核苷酸可显著降低肥胖大鼠的体脂、血脂 ,其机制可能与腹膜后脂肪组织

关 键 词：adrenergic receptor OBESITY gene therapy receptor  neuropeptide Y 

分 类 号：R589.2[医药卫生—内分泌] R459.9[医药卫生—内科学]