Detection of the SYT-SSX Fusion Gene in Synovial Sarcoma  

滑膜肉瘤融合基因SYT-SSX检测(英文)

作　　者：杨翎[1] 范钦和[1] 张炜明[1] 

机构地区：[1]南京医科大学第一附属医院病理科

出　　处：《Journal of Nanjing Medical University》2004年第1期36-39,共4页南京医科大学学报（英文版）

摘　　要：Objective: To investigate the feasibility and significance of detecting SYT-SSX fusion gene in paraffin-embedded tissues of synovial sarcoma (SS) by reverse-transcriptase polymerase chain reaction(RT-PCR) methods. Methods: Twenty cases of SS tumors from archival materials were collected and all samples were formalin-fixed and paraffin-embedded (FFPE). SYT-SSX fusion transcript was detected by RT-PCR. Home-keeping gene Porphobilinogen Deaminase (PBGD) was regarded as internal control.Results: PBGD mRNA was detected in all 20 tumor cases (100%). SYT-SSX fusion transcript was detected in 18 tumor cases (90%). In 18 SYT-SSX positive SS cases, there are 12 present SYT-SSX1 fusion transcript and 6 present SYT-SSX2 fusion transcript. SYT-SSX1 fusion transcript can be seen in 9 monophasic SS and 3 biphasic SS. In 6 SYT-SSX2 positive SS cases, 4 were monophasic SS and 2 were biphasic. Conclusion: Detection of SYT-SSX fusion transcripts in FFPE tissues for diagnosis of SS is feasible and sensitive. Subtypes of SYT-SSX fusion gene may provide prognosis information.目的 :研究采用逆转录 -聚合酶链反应的方法在滑膜肉瘤石蜡包埋组织中检测SYT SSX融合基因的可行性和临床意义。方法 :收集 2 0例滑膜肉瘤标本 ,均为甲醛固定 ,石蜡包埋组织。SYT SSX融合基因采用逆转录 -聚合酶链反应 (RT PCR)检测。看家基因PBGD作为内参照。结果 :所有标本均可检测到PBGDmRNA的表达。 18例检测到融合基因SYT SSX的表达 ,其中 ,SYT SSX1型占 12例 ,SYT SSX2型占 6例 (4例为单相型滑膜肉瘤 )。结论 :在滑膜肉瘤甲醛固定 ,石蜡包埋组织中用RT PCR方法检测SYT SSX融合基因是可行的 ,有较高的敏感性。融合基因亚型与滑膜肉瘤组织学分型有一定关系 。

关 键 词：synovial sarcoma SYT-SSX fusion gene RT-PCR 

分 类 号：R738.5[医药卫生—肿瘤]