双探针原位杂交法检测慢性肝病和肝细胞癌患者肝组织TTV DNA的感染状况  

Assay of in situ hybridization with double probes for TTV DNA status in liver tissues of patients with chronic liver diseases and hepatocellular carcinoma

作　　者：邓传珍[1] 姜岭梅[1] 李银鹏[1] 朱惠明[1] 

机构地区：[1]深圳市人民医院消化内科,广东省518020

出　　处：《实用肝脏病杂志》2004年第1期16-18,共3页Journal of Practical Hepatology

基　　金：广东省深圳市科技计划项目课题 (970 0 86)

摘　　要：目的　探讨慢性肝病和肝细胞癌患者肝组织TTVDNA的感染状况。方法　采用PCR扩增法分别合成G1a、G2b两种亚型的双链TTVDNA探针。应用两型探针对 45例肝组织标本进行TTVDNA原位杂交检测 ,巢氏PCR法检测血清TTVDNA。结果　 3 1例血清TTVDNA阳性患者的肝组织TTVDNA均为阳性 ( 10 0 % )。 14例血清TTVDNA阴性的患者肝组织中TTVDNA阳性者 7例 ( 5 0 % )。慢性肝病患者的肝组织中TTVDNA散在分布在汇管区周围的肝细胞核内 ,肝癌患者TTVDNA则集中分布在肝癌细胞核内及癌组织周围的肝细胞核内。结论　慢性肝病与肝癌患者肝组织中TTVDNA的感染状态存在一定差异。Objective To investigate transfusion transmitted virus (TTV) DNA status in the liver tissues of patients with chronic liver diseases and hepatocellular carcinoma. Methods Double probes of TTV DNA, genotype 1a and 2b,were synthesized by means of polymerase chain reaction (PCR) , respectively. Liver tissues were tested by in situ hybridization with double probes for 45 patients with chronic liver diseases and hepatocellular carcinoma. Sera were tested by nested-PCR. Results TTV DNA in liver tissues was positive in 31 patients with positive serum TTV DNA(100%), whereas TTV DNA in liver tissues was positive in 7(50%) of 14 patients with negative serum TTV DNA. TTV DNA was separately distributed in the nucleolus of hepatic cells around portal area in patients with chronic live diseases. In contrast to patients with chronic liver diseases, TTV DNA was mainly distributed in the nucleolus of hepatocellular carcinoma and hepatic cells around cancer tissue in patients with hepatocellular carcinoma.Conclusion Infectious status of TTV DNA in the liver tissues is different in patients with chronic liver diseases and hepatocellular carcinoma.

关 键 词：双探针原位杂交法 检测 慢性肝病 肝细胞癌 肝组织 TTV DNA感染 

分 类 号：R735.7[医药卫生—肿瘤] R512.6[医药卫生—临床医学]