水-离子液和水-有机溶剂体系中萘普生酶法拆分的比较研究  

The Comparison Study on the Enzymatic Resolution of Naproxen in Aqueous-Ionic Liquids with Aqueous-Organic Solvent System

作　　者：赵永杰[1] 辛嘉英[1] 李臻[1] 夏春谷[1] 李树本[1] 

机构地区：[1]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《分子催化》2004年第1期6-9,共4页Journal of Molecular Catalysis(China)

基　　金：国家自然科学基金重点项目(29933040);国家重点基础研究规划项目.

摘　　要：在水-有机溶剂和水-离子液两相体系中研究了脂肪酶催化的萘普生甲酯的立体选择性水解反应.考察了转化率,对映体过量值(eep);(ees)与时间的关系.据此构建了一种可以进行萘普生甲酯立体选择性水解的水-离子液两相体系.在该水-离子液两相体系中酶的活性与传统的水-有机相两相体系相比没有明显的变化,但是酶的立体选择性却明显提高.同时也对水-离子液两相体系中水含量对萘普生甲酯立体选择性水解反应的影响进行了研究,发现在水∶离子液(v/v)为1∶1时酶的活性和立体选择性最好.In this paper, lipase-catalyzed steroselective hydrolysis of the racemic Naproxen methyl ester was performed in aqueous-organic biphase system and aqueous-ionic liquid biphase system respectively. The relationship between conversion(C), substrate/product enantiomeric excess(ee\-p/ee\-s)and the reaction time was examined. An aqueous-ionic liquid biphase system for the steroselective hydrolysis of the racemic Naproxen methyl ester was developed. In this system, the activity of the lipase was as good as that in the traditional aqueous-organic biphasic system. But the enantioselectivity of the lipase in the aqueous-ionic liquid biphase system was higher than that in the traditional aqueous-organic biphasic system. Also, effect of the water content on the lipase activity and enantioselectivity was studied. The best activity and enantioselectivity have been obtained when the ratio of aqueous phase to ionic liquid phase equals to 1.

关 键 词：萘普生 立体选择性水解 水-离子液两相体系 脂肪酶 

分 类 号：O643.32[理学—物理化学]