血红素氧合酶-1对大鼠脑缺血再灌注后血脑屏障通透性的影响  

AEffect of hemeoxygenase 1 on permeability of blood brain barrier after cerebral ischemia and reperfusion in rats

作　　者：冯新红[1] 袁伟[2] 沈霞[1] 

机构地区：[1]徐州医学院附属医院神经内科,江苏省徐州市221002 [2]徐州医学院附属医院创伤外科,江苏省徐州市221002

出　　处：《中国临床康复》2003年第31期4188-4189,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨血红素氧合酶(hemeoxygouase-1,HO-1)对大鼠局灶性脑缺血再灌注后血脑屏障(bloodbrainbarrier,BBB)通透性的影响。方法:健康成年雄性SD大鼠,体质量250～300g,采用线栓法制备局灶性脑缺血模型,通过免疫组化观察脑组织HO-1的表达,通过比色法测定脑组织中伊文思蓝(EB)的含量来反映血脑屏障通透性的改变。结果:缺血2h再灌注2h即见脑缺血区的周围大脑皮质及远隔区域有HO-1蛋白的表达,免疫阳性持续到再灌注后48h。缺血2h再灌注3hEB含量(1.36±0.10)g/L,BBB通透性开始增加,24h达高峰(5.64±1.30)g/L,48h后开始减弱。Znpp组EB含量(6.53±0.02)g/L较缺血2h再灌注24h增高。结论:脑缺血再灌注后HO-1快速高效表达,可能对BBB有保护作用。AIM:To explore the effect of hemeoxygenase(HO) 1 on the permeability of blood brain barrier(BBB) during reperfusion after focal cerebral ischemia. METHODS:Healthy adult male SD rats,weighed 250-300 g, were made into models of focal cerebral ischemia with thread occlusion method.The expression of HO 1 protein in brain tissue was observed by immunohistochemistry,the change of the permeability of BBB was reflected by the content of evans blue(EB)in brain tissue measured by colorimetric method. RESULTS:Expression of HO 1 protein was immediately observed within 2 hours of reperfusion and 2 hours after cerebral ischemia in the cerebral cortex around and far from the area of cerebral ischemia,immune positive sign lasted until 48 h after reperfusion.The content of EB was [(1.36±0.10) g/L] when ischemia for 2 h and reperfusion for 3 h,and the permeability of BBB began to increase,and reach the peak at the 24[(5.64±1.30) g/L] and began to decreased at the 48 h.The content of EB in Znpp group[(6.53±0.02) g/L] was higher than that of when ischemia 2 h and reperfusion 24 h. CONCLUSION:HO 1 protein of focal cerebral ischemia and after reperfusion express immediately and effectively, and may play a protective role in blood brain barrier.

关 键 词：血红素氧合酶-1 大鼠 脑缺血 再灌注治疗 血脑屏障 通透性 免疫组化 

分 类 号：R743[医药卫生—神经病学与精神病学]