血管性痴呆大鼠海马突触结构参数的变化(英文)  

Structural changes in the hippocampal synapse of rats with vascular dementia

作　　者：赵小贞[1] 王玮[1] 康仲涵[1] 吴翊钦[2] 徐剑文[1] 陈春鹏[1] 钟秀容[2] 

机构地区：[1]福建医科大学基础医学院解剖与组胚学系,福建省福州市350004 [2]福建医科大学电镜室,福建省福州市350004

出　　处：《中国临床康复》2003年第31期4213-4215,共3页Chinese Journal of Clinical Rehabilitation

基　　金：福建省教育厅科研基金资助项目(JA00196)~~

摘　　要：目的:观察血管性痴呆大鼠海马突触结构特点,探讨血管性痴呆学习记忆障碍的神经病理机制。方法:采用永久性结扎双侧颈总动脉的方法建立血管性痴呆动物模型,利用Morris水迷宫和Y型电迷宫检验大鼠的学习记忆能力,应用透射电镜和形态计量法分析血管性痴呆大鼠海马GrayⅠ型突触结构参数的变化。结果:血管性痴呆大鼠海马CA1区GrayΙ型突触的体积密度、面积密度、比表面、面数密度、突触界面曲率、突触间隙宽度和突触后致密物厚度分别为犤0.051±0.016,(0.298±0.130)μm2/m3,(5.884±1.203)μm2/m3,(3.285±1.113)个/85.05μm2,1.048±0.060,(13.97±0.386)nm和(33.33±0.86)nm犦,比假手术对照组犤分别为0.088±0.019,(0.712±0.815)μm2/m3,(9.020±1.915)μm2/m3,(6.330±1.258)个/85.05μm2,1.103±0.268,(25.40±0.92)nm和(47.73±1.15)nm犦减小,血管性痴呆大鼠与假手术对照组突触平均面积分别为(1.573±0.343)m2和(1.202±0.145)m2。结论:永久性结扎双侧颈总动脉使大鼠海马CA1区GrayΙ型突触数量减少和形态结构发生显著变化,导致学习记忆障碍。AIM:To explore the neuropathological mechanism of vascular dementia(VD) related learning and memory disorder by observing the structural changes of hippocampal synapses in VD rat. METHODS:Rat VD models established by permanent occlusion of the bilateral common carotid artery(POBCCA) underwent Morris water maze and Y maze to assess their learning and memory ability.Structural alternations of Gray typeⅠsynapse of the hippocampus in VD rats were analyzed by transmission electron microscopy and morphological measurement. RESULTS:Structural parameters of Gray typeⅠsynapse in hippocampal CA1 region of VD rats were measured,including the volume density,area density,specific surface area,surface density,synaptic interface curvature,intersynaptic space wideness and postsynaptic density thickness[0.051±0.016,(0.298±0.130) μm2/m3,(5.884±1.203) μm2/m3,(3.285±1.113)/85.05 μm2,1.048±0.060,(13.97±0.386) nm and (33.33±0.86) nm respectively],which were significantly reduced as compared with those in sham operation group[0.088±0.019,(0.712±0.815) μm2/m3,(9.020±1.915) μm2/m3,(6.330±1.258)/85.05 μm2,1.103±0.268,(25.40±0.92) nm and (47.73±1.15) nm, respectively].Synaptic average area in the rats in VD group and sham operation group was (1.573±0.343) μm2 and (1.202±0.145) μm2 respectively. CONCLUSION:Significantly decreased quantity and structural changes of Gray type Ⅰsynapses occur in the hippocampal CA1 region of VD rats after permanent occlusion of the bilateral common carotid arteries,which may induce learning and memory disturbance.

关 键 词：血管性痴呆 大鼠 海马突触结构参数 学习记忆障碍 

分 类 号：R749.13[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]