8-氯腺苷抑制肿瘤坏死因子α诱导的视网膜细胞原癌基因表达的实验研究  

The inhibitory effect of 8-chloroadenosine on tumor necrosis factor-induced proto-oncogene expression of retinal pigment epithelial cells

作　　者：杨萍[1] 张惠蓉[1] 

机构地区：[1]北京大学第三医院北京大学眼科中心,100083

出　　处：《中华眼科杂志》2003年第12期750-752,共3页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 (3 92 0 0 13 2 ) ;博士学科点专项科研基金资助项目 (95 0 10 3 8)

摘　　要：目的　探讨 8 氯腺苷 (8 CLA)抑制肿瘤坏死因子α(TNF α)诱导的视网膜色素上皮(RPE)细胞原癌基因的表达情况 ,为寻找抑制细胞增殖的新药提供依据。方法　取健康牛眼RPE细胞进行培养 ,采用Northern杂交法测定 8 CLA对TNF α诱导的RPE细胞c fos及c mycmRNA表达的影响。结果　正常培养的RPE细胞有少量c fos及c mycmRNA表达 ;加入TNF α实验组 ,RPE细胞中c fos及c mycmRNA表达量增加 ,分别为对照组的 3 1和 1 5倍 ;加入TNF α和 8 CLA实验组 ,经TNF α刺激的RPE细胞中c fos及c mycmRNA表达量降低 ,分别较未用药组下降 2 5 0 %和2 9 0 %。结论　 8 CLA可抑制TNF α诱导的RPE细胞c fos及c myc原癌基因的表达 ,此项研究结果将为临床探索有效抑制生长因子诱导的RPE细胞增殖的药物研究提供理论依据。Objective To investigate the inhibitory effect of 8-chloroadenodine (8-CLA) on tumor necrosis factor-α (TNF-α)-induced proto-oncogene expression in retinal pigment epithelial (RPE) cells. Methods Primary culture and subculture of health bovine RPE cells were established in vitro. RPE cells were treated with tumor necrosis factor TNF-α(13.3mmol/L) with or without 8-CLA (16 μmol/L) for 30 minutes or 3 hours, respectively. C-fos and c-myc mRNA expression in RPE cells was detected by Northern blot analysis. Results Normal RPE cells expressed c-fos and c-myc mRNA at a low level. After treatment with TNF-α, the expression of c-fos and c-myc mRNA was increased by 3.13 and 1.5 fold, respectively, as compared to untreated cells. 8-CLA decreased TNF-α-induced c-fos and c-myc mRNA expression by 25.0% and 29.0%, respectively. Conclusions 8-CLA can inhibit TNF-α-induced c-fos and c-myc mRNA expression in RPE cells. These findings may be useful for exploring new drugs for inhibiting the growth-induced proliferation of RPE.

关 键 词：8-氯腺苷 肿瘤坏死因子α 8-CLA TNF-α 视网膜细胞 原癌基因 基因表达 PVR 免疫组织 

分 类 号：R774.1[医药卫生—眼科]