小麦淀粉粒束缚淀粉合成酶基因多态性的分子鉴定  

Molecular Identification of Wheat Granule Bound Starch Synthase Gene Polymorphism

作　　者：宋建民[1] 李保云[1] 尤明山[1] 梁荣奇[1] 常成[1] 刘守斌[1] 唐朝晖[1] 刘广田[1] 

机构地区：[1]中国农业大学植物遗传育种系

出　　处：《Acta Genetica Sinica》2004年第1期81-86,共6页

基　　金：国家自然科学基金 (编号 :3 993 0 110 );国家 863计划 (编号 :2 0 0 1AA2 410 3 1;编号 :2 0 0 2AA2 0 70 0 3 )项目资助~~

摘　　要：运用 6 %的SDS PAGE对 14个小麦品种成熟籽粒Wx蛋白的多态性进行了鉴定 ,结果表明 ,14个小麦品种根据其Wx蛋白的缺失情况可分为 6种组合类型。另外 ,根据Wx A1、Wx B1和Wx D1这 3个位点基因序列和变异情况分别设计了PCR引物 ,扩增结果表明 :Wx A1位点突变材料扩增产物为 32 7bp ,正常材料中扩增不到该特异带 ;在Wx B1位点扩增出 187bp目标带 ,突变材料没有该扩增产物 ;在Wx D1位点扩增出约 70 0bp目标带 ,突变材料没有该特异带。与前人的研究结果相比 ,Wx B1引物在 3个位点的扩增产物长度更短 ,差异更大 ,在 2 %琼脂糖胶上即可清楚分开 ,缩短了鉴定时间 ,提高了效率 。Fourteen wheat cultivars were identified into six types of Wx proteins combinations using 6% SDS-PAGE.PCR primers were designed according to the three Wx genes sequences and their mutants,respectively.A 327 bp-band was amplified from the Wx-A1 mutant,while the band was absent for the normal alleles at the Wx-A1 locus,as well as the presence or absence of a 187 bp PCR fragment at the Wx-B1 locus and a 700 bp PCR fragment at the Wx-D1 locus,respectively,corresponding to the normal and mutant alleles.Compared with the former studies,shorter and more different PCR products at three loci,amplified by the primers designed for Wx-B1 gene can be separated in 2% agarose gel,which enables screening breeding lines for noodle use faster and effectively.

关 键 词：小麦 淀粉粒束缚淀粉合成酶基因 多态性 分子鉴定 

分 类 号：S512.101[农业科学—作物学]