急性前脑缺血致多器官功能障碍综合征动物模型的建立及内毒素受体CD14的表达  

Gene expression of endotoxin receptor CD14 in animal model of multiple organ dysfunction syndrome caused by acute forebrain ischemia

作　　者：郭洪志[1] 屈传强[2] 贾建平[3] 

机构地区：[1]济南山东大学齐鲁医院神经内科,250012 [2]济南山东大学齐鲁医院影像中心,250012 [3]首都医科大学宣武医院神经内科

出　　处：《中国脑血管病杂志》2004年第2期85-90,共6页Chinese Journal of Cerebrovascular Diseases

摘　　要：目的 探讨建立大鼠急性前脑缺血致多器官功能障碍综合征模型的可能性,研究脑缺血模型内毒素受体CD14 mRNA在肺、肝、肠和肾组织的表达变化规律与导致多器官功能障碍综合征(MODS)的发生机制。 方法 通过阻断双侧颈总动脉30 min建立急性前脑缺血模型,随机将54只大鼠分为正常对照组(6只)、假手术组(8只大鼠)及缺血后5个亚组(12、24、36、48及72 h组,每组8只大鼠),依据全身炎症反应综合征(SIRS)、MODS的诊断标准判断SIRS、MODS的发生率,采用原位杂交技术检测缺血后肺、肝、肠和肾脏器CD14的基因表达。 结果 (1)缺血后12 h肺、肝、肠和肾组织CD14 mRNA表达升高,24-36 h达高峰,48 h后下降,以肺脏变化最显著(P<0.001);(2)缺血后12、24、36、48及72 h时相点动物肺、肝、肠和肾组织均有不同程度的损害,但以24-48 h时脏器病理变化较显著,其中以肺脏改变为著,与CD14在各器官的基因表达峰值一致;(3)大鼠急性前脑缺血后SIRS发生率为100%,MODS发生率为53.1％;(4)正常对照组和假手术组中肺、肝、肠和肾组织CD14 mRNA均有不同程度的表达,其中两组肺脏CD14 mRNA的表达差异有显著意义(P<0.01)。结论 (1)大鼠急性前脑缺血可成功建立脑缺血致MODS的动物模型;(2)脑缺血致MODS动物模型的肺、肝、肠和肾各脏器CD14Objective To explore the possibility of establishing a animal model of multiple organ dysfunction syndrome (MODS) caused by acute forebrain ischemia; to investigate changes of endotoxin receptor CD14 gene expression in lung, liver, intestine, kidney tissues in model of cerebral infarction, and the pathogenesis of MODS caused by brain ischemia. Methods Model of cerebral infarction was established by 30 minutes of group(n=6)、sham-operative group(n=8) and 5 ischemia groups(n=40) including 12 h, 24 h, 36 h, 48 h, 72 h five bilateral common carotid artery occlusion. 54 Wistar rats were randomly divided into seven groups: normal control time points. Incidences of SIRS and MODS were diagnosed by their diagnostic criteria. Gene expression of CD14 was assayed using in situ hybridization. Results 1. The CD14 mRNA expression in lung, liver, intestine and kidney tissues increased after brain ischemia, reaching the peak at the 24h-36h, and decreased! after 48 hours. The highest level of CD14 mRNA expression was found in lung(P<0.001). 2. Injuries at each time point were observed in lung, liver, intestine, kidney tissues in varying degrees, and the lesion of lung was the most seri-ous. There was the consistency between CD14 mRNA expression and histological changes of each organ. 3. Incidences of SIRS and MODS were respectively 100% and 53. 1% after acute forebrain ischemia in rats. 4. A few of CD14mRNA expressed in each organ of normal control group. Compared with normal control group, the CD14 mRNA expression significantly elevated in pulmonary tissue of sham-operative group(P<0.01) . Conclusion 1. An experimental animal model of MODS can be established successfully through acute forebrain ischemia in rats. 2. The CD14 mRNA expression of each organ is significant elevated in model of MODS caused by brain ischemia. 3. Pathological changes of intestinal mucosa and liver tissues can provide conditions for occurrence of endotoxin translocation and endotoxemia. 4. endotoxin transloca-tion can cause directly MODS after brain isch

关 键 词：急性前脑缺血 多器官功能障碍综合征 动物模型 内毒素 受体 CD14 基因表达 

分 类 号：R743[医药卫生—神经病学与精神病学]