食管癌发生过程中抑癌基因PTEN表达与微量元素含量分析  

Expressions of suppressor gene PTEN and analysis of trace element content in the development of esophageal carcinoma

作　　者：高新平[1] 王建设[2] 朱命炜[2] 赵卫星[1] 王全智[3] 

机构地区：[1]新乡医学院病理教研室,河南新乡453003 [2]河南师范大学,河南新乡453002 [3]新乡医学院第三附属医院病理科,河南新乡453003

出　　处：《新乡医学院学报》2004年第1期5-7,共3页Journal of Xinxiang Medical University

基　　金：河南省科技攻关资助项目 (9612 0 0 10 2 )

摘　　要：目的　探讨 PTEN基因表达和微量元素变化与食管癌变的关系。方法　对 12 8例正常食管、单纯增生、非典型增生活检、食管癌切除标本 ,采用 X-射线能谱仪检测微量元素含量 ,S-P免疫组化染色法检测 PTEN表达变化。结果　随食管上皮增生程度加重 ,PTEN阳性表达与微量元素 Zn、Se、Mo、Ca、Mg含量渐减 ,Cu、Cu/ Zn含量递增 ,食管癌与正常食管上皮、单纯增生及非典型增生组间差异显著 (P<0 .0 5)。结论　食管上皮癌变过程中 PTEN基因表达有明显缺失现象 ,可能与 Zn、Mo、Ca、Se、Mg、Cu、Cu/ObjectiveTo discuss the relationship between express io ns of suppressor gene Phosphate and tensin homology deleted on chromosometen(PT EN) and trace element content during the development of esophageal squamous cell carcinoma.MethodsThe quantitative de termination of trace element of resection and biopsy samples in 128 cases of eso phageal carcinogenesis,normal,hyperplasia or dysplasia were performed by using X -ray energy spectrometry and the detection of PTEN gene expression by using imm munohistochemical staining.ResultsThe positive expression rate of PTEN and the content of Zn, Se, Mo, Ca, Mg were gradually reduced with advan cement of esophageal lesions,but the content of Cu, Cu/Zn was gradually increase d.Among normal esophageal epithelium, hyperplasia, dysplasia and esophageal carc inoma, the difference significant (P<0.05).ConclusionThe e xpression deletion of gene PTEN is obvious, and which is perhaps related to th e content variation of Zn, Se, Mo, Ca, Mg, Cu, Cu/Zn probably.

关 键 词：抑癌基因 PTEN 微量元素 食管癌 癌前病变 

分 类 号：R735.1[医药卫生—肿瘤]