高效液相色谱法测定豆粕中大豆异黄酮的含量  

DETERMINATION OF ISOFLAVONE FROM SOY RESIDUE BY HPLC

作　　者：徐颖[1] 董文宾[1] 张建华[1] 申延[1] 叶增民[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西咸阳712081

出　　处：《陕西科技大学学报（自然科学版）》2003年第4期79-82,共4页Journal of Shaanxi University of Science & Technology

摘　　要：采用超声波法提取豆油加工副产品豆粕中的大豆异黄酮,通过高效波相色谱法测定了其含量。在大量摸索性试验的基础上,确定出色谱条件为:色谱柱Waters RP_(18)(5μm,3.9mm×150mm),流动相甲醇:水:磷酸=52:48:0.2,柱温30℃,流速0.7mL/min,检测波长260nm。该测定方法的回收率为99.3％,变异系数小于3％。采用高效液相色谱法测定异黄酮的含量,具有灵敏、准确、重现性好等优点。A method of extracting isoflavone from soy residue by ultrasonic wave , determinating it by high performance liquid chromatography (HPLC) is described. The column is Waters Symmetry Column ( RP18,5um ,3. 9mmx150mm) The mobile phase is MeOH:H2O:H3PO4 (52 = 48:0.2), the temperature of column is 30C , the wavelength is 260nm,the flow rate is 0. 7mL/min. The average recovery rate is 99. 3% with RSD less than 3%. It is concluded that this method is senitive,accurate and reprodu-ciable.

关 键 词：豆粕 大豆异黄酮 高效液相色谱法 测定 超声波法 提取 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]