5-氟胞嘧啶对胞嘧啶脱氨酶基因修饰的胰腺癌细胞凋亡的诱导作用  

Apoptosis induced by 5-fluorocytosine in human pancreatic cancer cells genetically modified to express cytosine deaminase

作　　者：张世能[1] 袁世珍[1] 朱兆华[1] 文卓夫[1] 黄志清[1] 曾志勇[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院消化内科,广东广州510120

出　　处：《中国病理生理杂志》2001年第1期32-35,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨 5 -氟胞嘧啶 ( 5 -FC)对基因修饰的胰腺癌细胞凋亡的影响及特征。方法 :以腺病毒介导的胞嘧啶脱氨酶 (CD)基因转染胰腺癌SW1990细胞 ,以Westernblot检测目标基因蛋白水平表达 ,通过细胞形态学、脱氧核糖核酸 (DNA)凝胶电泳和流式细胞术观察 5 -FC对表达CD基因的SW1990细胞凋亡的影响作用。结果 :以含CD基因的重组腺病毒转染的SW1990细胞 ,给予 5 -FC10 0 μmol·L-1,培养 4 8h ,细胞出现典型的凋亡形态、DNA梯形改变及凋亡峰 ,细胞在G1,S和G2 /M各期分别为 64%、11%和 7%,凋亡率达 3 4 .6%。结论 :5 -FC的上述诱导凋亡作用可能是胰腺癌CD基因疗法的重要机制。AIM: To elucidate the pattern of 5-fluorocytosine(5-FC) induced apoptosis and its role in gene therapy for human pancreatic cancer. METHODS: The human pancreatic cancer SW1990 cells(CEA-producing) were infected with recombinant adenoviruses(Adex1CEA-prCD or Adex1CEA-prZ).Cytosine deaminase(CD) expression was examind by western blot. Apoptosis induced by 5-FC in human pancreatic cancer SW1990 cells genetically modified to express cytosine deaminase was investigated by applying electron microscopy, DNA electrophoresis and flow cytometry analysis techniques. RESULTS: The SW1990 cells infected with Adex1 CEA-prCD were treated with 5-FC at 100 μmol·L -1 for 48 h, cell apoptosis occurred. Typical apoptosis morphological feature appeared and DNA ladder could be demonstrated on DNA electrophoresis. Apoptosis peak was also showed by flow cytometry. Apoptotic cells accounted for 34.6% of the cell population. Cells in G 1, S and G 2/M phase of cell cycle were 64%, 11% and 7%, respectively. CONCLUSION: The apoptosis induced by 5-FC may be a primary mechanism in CD gene therapy for pancreatic cancer.

关 键 词：胰腺肿瘤 基因 凋亡 氟胞嘧啶 

分 类 号：R735.9[医药卫生—肿瘤]