功能矫形前伸下颌后大鼠髁突软骨细胞睾酮的分布变化规律  

Expression of Testosterone in the Rapid Growing Rat Condylar Cartilage and the Expression Changes after the Function Mandibular Advacement

作　　者：李小兵[1] 陈开云[1] 周征[1] 罗颂椒[1] 

机构地区：[1]四川大学华西口腔医院正畸科,四川成都610041

出　　处：《华西口腔医学杂志》2004年第1期29-31,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (39470 760 )

摘　　要：目的　研究快速生长期大鼠髁突软骨细胞睾酮的表达 ,并探讨功能矫形对髁突软骨细胞睾酮表达的影响。方法　实验组戴自制模拟的功能矫治器引导大鼠下颌前伸 ,在实验第 3天、1、2、3及 4周处死大鼠 ,应用免疫组织化学SP法检测睾酮在髁突软骨细胞上的表达特点。结果　①实验组、对照组大鼠髁突软骨细胞各层都有睾酮的阳性免疫染色 ,大鼠各层细胞中以成熟层及移行层阳性强度较高 ,明显高于生发层及过渡层 ,其中成熟层染色强度最高 ;②随着大鼠的青春期生长发育 ,髁突软骨细胞各层睾酮染色强度出现不同的变化特点 ;③实验第 2及第3周组大鼠髁突成熟层软骨细胞睾酮染色强度明显高于对照各组 ,差异具有统计学意义 (P <0 0 5 )。结论　睾酮参与了大鼠髁突软骨细胞成熟的调控 ,并介导了功能矫形的机械刺激作用 ,使髁突软骨细胞的增生分化功能增强 。Objective To investigate the expressions of Testosterone in the condylar cartilage of rapid growing rats.Methods The mimic functional appliances were used in experiment groups and rats were killed after 3 days , 1week, 2weeks, 3weeks and 4weeks. Immunohistochemical technique was used to detect the expression of T.Results T was expressed throughout the condylar cartilage. The Highest expressions were in mature layers of both experiment and control groups. Along with the maturation, the expressions of T in the condylar cartilage were changed respectively, and the immunostain levels in the mature layer of experiment groups 2 and 3 weeks were higher than those of control groups (P<0 05).Conclusion ①T is a member of cartilage growth and development control system. ②The orthopedic mechanism of functional appliance may be conducted by T.

关 键 词：功能矫形 前伸下颌 大鼠 髁突软骨细胞 睾酮表达 性激素 

分 类 号：R783.5[医药卫生—口腔医学]