快速蛋白液相色谱法纯化威廉环毛蚓纤溶酶及其活性研究  

Purification by FPLC and Activity of Fibrinolytic Enzyme from Pheretima Guillemi

作　　者：方成[1] 何执中[1] 朱锋荣[1] 施媚[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2004年第1期45-48,共4页Pharmaceutical Biotechnology

摘　　要：摸索威廉环毛蚓纤溶酶的分离纯化工艺和研究蚯蚓纤溶酶 (earthwormfibrinolyticenzyme ,EFE)体内外的抗栓溶栓活性。通过提取 ,分步盐析 ,再经AKTAbasic蛋白快速液相色谱进一步纯化 ,得一组有纤溶活性的同工酶 ,其中一种比活较大达 2 0 0 0IU/mg。药理试验表明该酶有明显的抗拴溶栓活性。To deter mine the purification process of the earthworm fibrinolytic enzyme of Pheretima guillelmi and to study its activity of inhibiting and dissolving thrombus,The earthworm fibrinolytic enzyme is purified by following steps: extracting, graded salting-out and FPLC(fast protein liquid chromatography).Several isoenzymes was obtained,one of them is more active than others, the specific activity of which reached 2 000 IU/mg. The pharmacological experiments showed that the enzyme can inhibit thrombosis and dissolve chrombus effectively.

关 键 词：纤溶酶 分离纯化 蛋白快速液相色谱 

分 类 号：Q51[生物学—生物化学]