舒林酸对胃癌细胞株SGC7901细胞周期调控及凋亡的影响  

Cell cycle arrest and apoptosis induction of sulindac on human gastric cancer cells SGC7901

作　　者：李乐[1] 赵玉元[1] 郭久冰[1] 高明太[1] 姚南[1] 陈健[1] 赵成基[1] 靳曙光[1] 

机构地区：[1]兰州医学院附属第一医院小儿外科,兰州730000

出　　处：《实用肿瘤学杂志》2004年第1期5-7,共3页Practical Oncology Journal

摘　　要：目的　观察舒林酸影响胃癌细胞株SGC790 1细胞周期分布 ,诱导细胞凋亡 ,探讨其作用机制方法　采用MTT比色法观察舒林酸对胃癌SGC790 1细胞增殖的影响 ,采用流式细胞仪检测舒林酸对细胞周期分布的影响 ,同时结合透射电子显微镜观察舒林酸诱导SGC90 1细胞凋亡的作用 ;免疫细胞化学方法观察舒林酸对胃癌细胞周期调控蛋白cyvclinE及P2 1WAF/CIPI的影响结果　舒林酸可抑制胃癌SGC790 1细胞的增殖 ,改变细胞周期分布 ,使G0 /G1期细胞比例增高 ,S期比例降低 ,并对SGC790 1细胞有促凋亡作用。上述作用具有剂量和时间依赖性 (P <0 .0 5 )。舒林酸还可上调P2 1WAF/CIPI蛋白表达。下调cyclinE蛋白表达。结论　舒林酸可改变细胞周期分布 ,影响细胞周期调控蛋白表达 ,并可诱导SGC790 1细胞凋亡 。Objective To investigate the effects of sulindac on proliferation and apoptosis of gastric cancer cells SGC7901 and its antineoplastic mechanism.Methods MTT assay was used to examine the effect of sulindac on the proliferation of SGC7901 cells; flow cytometry was used for the cell cycle distribution and estimating apoptosis; the morphology of apoptosis cells was investigated by means of Transmission electron microscopy. The expressions of cyclinE and P 21 protein were observed by immunocytochemical method.Results Sulindac inhibited the proliferation of SGC7901 cells, increased the proportion of cells in the G0/G1 phase and decreased the proportion in the S phase of cell cycle, and induced apoptosis of the SGC7901 cells line, the effects were of a time and concentration dependence. The expressions of cyclinE decreased while the expressions of P 21WAF/CIPI increased following treatment with sulindac.Conclusion Sulindac inhibits proliferation of SGC7901 cell line via induction of apoptosis and cell cycle arrest. The increased expression of P 21WAF/CIPI and the decreased expression of cyclinE may contribute to the cell cycle arrest following treatment with sulindac.

关 键 词：舒林酸 胃癌 SGC7901细胞 细胞周期 细胞凋亡 MTT比色法 流式细胞仪 细胞增殖 非甾体类抗炎药 

分 类 号：R735.2[医药卫生—肿瘤]