胃缺血/再灌注损伤引起大鼠脑室旁核及孤束核c-fos表达  

c-fos EXPRESSION WITHIN PVN AND NTS OF THE RAT INDUCED BY GASTRIC ISCHEMIA/REPERFUSION INJURY

作　　者：张咏梅[1] 张建福[1] 陈幽婷[2] 阎长栋[1] 周秀萍[1] 

机构地区：[1]徐州医学院生理学教研室,江苏徐州221002 [2]徐州医学院解剖学教研室,江苏徐州221002

出　　处：《中国应用生理学杂志》2004年第1期20-23,T001,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助课题 (3 9970 2 81) ;江苏省教育厅自然科学基金资助项目 (99KJB3 10 0 0 5 )

摘　　要：目的 :观察电和化学刺激下丘脑室旁核 (PVN)对大鼠胃缺血 /再灌注损伤 (GI/RI)的影响及孤束核 (NTS)在其中的作用和GI RI后PVN、NTS内c fos表达的情况。方法 :夹闭大鼠腹腔动脉 30min ,松开动脉夹血流复灌 1h制备GI/RI模型 ,应用Fos免疫组织化学法 (ABC法 )观察GI RI后中枢神经系统内c fos表达的情况。结果 :①电和化学刺激PVN后 ,GI/RI减轻。②损毁双侧NTS后 ,能取消电刺激PVN对GI RI的影响。③GI RI能引起PVN、NTS等核团内c fos表达增加。结论 :GI RI的伤害性刺激影响了PVN、NTS的功能。PVN、NTS参与了对GI RI的调控。Aim: To investigate the effect of paraventricular nucleus (PVN) stimulation and the c-fos expressione within PVN and nucleus tractus solitarius (NTS) of the rat following gastric ischemia/reperfusion injury (GI/RI). Methods: The rat celiac artery was clamped for thirty minutes and reperfused for sixty minutes,using Fos immunohistochemical method (ABC method) examined the c-fos expression within PVN and NTS. Results: ① Both electrical and chemical stimulation of the PVN obviously attenuated the GI/RI. ② Bilateral electrolytic lesion of NTS could elimilate the protective effect of electrical stimulation of the PVN. ③The Fos-like immunoreactive neurons were increased in bilateral PVN and NTS by GI/RI. Conclusion: The function of PVN and NTS could be affected by the GI/RI noxious stimulation. PVN,NTS were involved in the regulation of GI/RI.

关 键 词：胃缺血/再灌注损伤 室旁核 孤束核 下丘脑 

分 类 号：R743.3[医药卫生—神经病学与精神病学]