利用基因芯片研究六味地黄丸对老年大鼠基因表达的影响  

Effect of Liuwei Dihuang Wan on the expression of genes in aged rats by cDNA microarray

作　　者：余榕捷[1] 蒲含林[1] 周天鸿[1] 李志英[1] 林剑[1] 

机构地区：[1]暨南大学生物工程研究所,广东广州510632

出　　处：《中国病理生理杂志》2004年第2期260-265,共6页Chinese Journal of Pathophysiology

摘　　要：目的 :利用基因芯片研究六味地黄丸对老年大鼠基因表达的影响。方法 :将 4 0只 2 0月龄SD雄性大鼠分成不用药与用六味地黄丸两组 ,用药 5周后 ,通过检测相关生化指标 ,确定用药组与对照组之间具显著差异。提取 2 0只 4月龄SD雄性大鼠脾脏mRNA ,平均分成两组 ,分别与老年用药组脾脏mRNA和老年对照组脾脏mRNA配对进行逆转录和芯片杂交实验 ,获得老年对照组和老年用药组分别和青年组相比 ,相关基因表达水平的差异 ,通过比较两组数据 ,评价六味地黄丸对与衰老相关的基因表达的调节作用。结果 :芯片结果显示老年对照组与青年组相比有 13个基因表达显著下调 ,1个基因表达显著上调。而在老年用药组中 ,14个相关基因的表达水平没有明显变化 ;而另有 2序列与青年组相比表达显著上调。结论 :通过基因芯片技术 ,发现 16个基因的表达水平可受六味地黄丸用药的影响。通过深入研究对我们深入理解六味地黄丸抗衰老的分子机制可能有帮助。AIM: To study the effect of Liuwei Dihuang Wan (LWDHW) on the expression of immune-associated genes in the aged rats using cDNA microarray. METHODS: Forty 20-months-old rats were divided into two groups equally. One group was treated with LWDHW for 5 weeks,another was untreated as control. Some biochemical assays were used to confirm the physiological differences between two groups. The mRNA from the spleens of twenty 4-months-old young rats were extracted and divided equally into two parts. Each part was conjoined with mRNA from drug treated aged rats and that from untreated control aged rats,respectively,and two experimental combinations for reverse transcription and cDNA microarray were formed. By comparing two groups of data from two combinations,the effects of LWDHW on the expression of the associated genes were evaluated. RESULTS: 13 down-regulated genes and one up-regulated gene were identified in the untreated control old rats,whose expression did not change obviously in treated old rats compared with young rats. The expressions of another two sequence were up-regulated distinctly in treated old rats compared with young rats. CONCLUSIONS: 16 immune-associated genes expressions were affected markedly by LWDHW. It will be helpful for understanding the molecular mechanism of LWDHW in ageing.

关 键 词：六味地黄丸 寡核苷酸序列分析 基因表达 衰老 

分 类 号：R363[医药卫生—病理学]