人抑癌基因PTEN对前列腺癌细胞系生物学行为的影响  

Adenoviral-mediated expression of human tumor suppressor gene PTEN inhibits proliferation and invasive ability of human prostate cancer cell lines LNCaP and DU-145

作　　者：孙颖浩[1] 邱镇[1] 许传亮[1] 钱松溪[1] 王元天[1] 沈茜[2] 李云[1] 唐楠[1] 

机构地区：[1]第二军医大学附属长海医院泌尿外科,上海200433 [2]第二军医大学附属长海医院中心实验室,上海200433

出　　处：《中华泌尿外科杂志》2004年第2期84-87,共4页Chinese Journal of Urology

基　　金：国家自然科学基金-重大国际合作研究项目( 30 1 1 0 6 6 5);全军医学科学技术研究"十五"计划重点课题 ( 0 1Z0 6 7)

摘　　要：目的　探讨外源性人抑癌基因PTEN表达对前列腺癌细胞系LNCaP、DU 14 5细胞增殖和侵袭转移能力的影响。　方法　利用携带人PTEN基因的可调控性腺病毒 (Ad PTEN ) ,体外转染人前列腺癌细胞系LNCaP、DU 14 5,RT PCR、Westernblot检测目的基因不同水平的表达 ,通过细胞生长试验、流式细胞仪分析技术以及Boyden小室法检测LNCaP、DU 14 5转染前后细胞增殖、细胞周期、细胞凋亡和细胞体外侵袭力的变化。　结果　转染Ad PTEN后LNCaP、DU 14 5细胞的PTEN表达由阴性转为阳性 ,转染后对LNCaP、DU 14 5细胞生长有抑制作用 ,阻滞于G0 ～G1期 ,早期细胞凋亡率增加 ,LNCaP细胞 (6.89± 0 .51) % ;DU14 5细胞 (5.44± 1.13 ) % ,与对照组相比差异有显著性 ,Boyden小室法检测转染后体外侵袭力受到明显抑制。 　结论　重组腺病毒介导的人PTEN基因在体外对前列腺癌细胞系LNCaP、DU 14 5的细胞增殖和体外侵袭力有明显抑制作用 。Objective To evaluate the effects of exog en ous adenovirus-mediated gene transfer of human tumor suppressor gene PTEN (phos phatase and tensin homology deleted on chromosome 10) on cell prolifiration and invasive ability of human prostate cancer cell lines LNCaP and DU-145. Methods mRNA and protein expressions of PTEN in human prostate c ancer LNCaP and DU-145 cells infected with AdPTEN were detected by RT-PCR and Western blot.MTT assay was used to assess the effect of PTEN on cell proliferati on.The change of cell cycle and apoptosis were measured by flow cytometry and th e invasion abilities in vitro detected by Boyden chamber.Ad-X-TRE-βgal v irus was used as control. Results RT-PCR and Western bl ot analysis showed that LNCaP and DU-145 cells express high level of PTEN after its infected with Ad-PTEN. The growth of LNCaP and DU-145 cells treated with Ad-PTEN was significantly more inhibited than that with a control adenovirus.Ad -PTEN-infected LNCaP and DU-145 cells showed a significant increase in G 0～ G 1 phase and PTEN-induced apoptosis in comparison with the control.The invasiv e ability of prostate cancer cell lines was inhibited by expression of PTEN effe ctively. Conclusions Adenoviral-mediated expression of human tumor suppressor gene PTEN has inhibitory affect on proliferation and inva sive ability of human prostate cancer cell lines LNCaP and DU-145.This study pr ovides an idea for the further development of Ad-PTEN as a potential therapeuti c vector in the gene therapy of prostate cancer.

关 键 词：抑癌基因 PTEN 前列腺癌 生物学行为 癌细胞系 

分 类 号：R737.25[医药卫生—肿瘤]