大鼠急性前脑缺血致多器官功能障碍综合征模型的建立  

Establishment of a model on acute forebrain ischemia complicated by multiple organ dysfunction syndrome (MODS)

作　　者：郭洪志[1] 屈传强[1] 

机构地区：[1]山东大学齐鲁医院神经内科,山东250012

出　　处：《脑与神经疾病杂志》2004年第1期11-13,共3页Journal of Brain and Nervous Diseases

摘　　要：目的:建立大鼠急性前脑缺血致多器官功能障碍综合征(MODS)模型,为探讨急性脑血管病(ACVD)并发MODS的发生机制奠定基础。方法:通过阻断双侧颈总动脉30分钟建立急性前脑缺血模型,随机将54只大鼠分为正常对照组、假手术组及缺血后五个亚组(12h、24h、36h、48h、72h组),记录大鼠缺血后各时相点的症状、体征及体温、呼吸变化,检测外周血WBC及肝、肾功能状态,光镜下观察肺、肝、肠和肾组织的病理变化,依据全身炎症反应综合征(SIRS)、MODS的诊断标准判断SIRS、MODS的发生率。结果:1.65％的缺血动物出现不同程度的腹胀,缺血组中动物的体温、呼吸、ALT、BUN及Cr变化与正常组、假手术组相比有显著性差异;并且在24h～48h之间变化最大;2.大鼠前脑缺血后各时相点动物的肺、肝、肠和肾组织均有不同程度的损害,24h到4Rh时相点的脏器病理变化较显著,72h时相点有所减轻;其中以肺脏的病理改变为著;3.大鼠急性前脑缺血后SIRS发生率为100％,MODS发生率为53.1％。结论:大鼠急性前脑缺血可成功建立脑缺血并发MODS的动物模型;急性肠道粘膜和肝组织的病理改变,为肠道内毒素易位和内毒素血症的发生提供条件。Objective: To establish a model of acute forebrain ischemia complicated by multiple organ dysfunction syndrome (MODS) , and contribute to the pathogenesis of acute cerebrovascular diseases ( ACVD) complicated by MODS. Methods: Model of cerebral infarction was established by 30minutes of bilateral common carotid artery occlusion. 54 Wistar rats were randomly divided into seven groups: normal control group( n = 6) , sham-operative group( n = 8) and 5 ischemia groups( n =40) including 12h, 24h, 36h, 48,72h five time points. Changes of symptoms and signs including temperature and respiration of each group were recorded. Pathological changes of lung、liver、 intestines and kidney were recorded through optical microscope. Incidences of SIRS and MODS were diagnosed by their criteria. Results: 1. 65% rats of operative groups had abdominal distention in varying degrees. There were significant differences in between operative group and normal control group, between operative group and sham-operative group. 2. Injuries at each time point were observed in lung, liver, intestines, kidney tissues in varying degrees, and lesion of lung was the most serious. There was the consistency between CD 14 mRNA expression and histological changes of ecah organs. 3. Incidences of SIRS and MODS were respectively 100% and 53. 1% after acute forebrain ischemia in rats. Conclusions: An experimental animal model of MODS can be established successfully through acute forebrain ischemia in rats Pathological changes of intestinal mucosa and liver tissues can provide conditions for occurrrence of endotoxin translocation and endotoxemia.

关 键 词：大鼠 急性前脑缺血 多器官功能障碍综合征 动物模型 全身炎症反应综合征 肠道内毒素易位 内毒素血症 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]