RAPD analysis of alfalfa DNA mutation via N^+ implantation  

作　　者：LIYu-Feng HUANGQun-Ce LIANGYun-Zhang YUZeng-Liang 

机构地区：[1]KeyLaboratoryoflonBeamBioengineering,InstituteofPlasmaPhysics,theChineseAcademyofSciences,Hefei230031 [2]InstttuteofBtology,XiangtanNormalUniversity,Xiangtan411201 [3]PhysicalDepartment,InnerMongoliaUniversity,Huhehaote010021

出　　处：《Nuclear Science and Techniques》2003年第3期161-163,共3页核技术（英文）

基　　金：Supported by Chinese Importance Science Foundation(No.119890300 and Chinese Natural Science Foundation(No.10065001)

摘　　要：Germination capacity of alfalfa seeds under low energy N+ implantation manifests oscillations goingdown with dose strength. From analyzing alfalfa genome DNA under low energy N+ implantation by RAPD (RandomAmplified Polymorphous DNA), it is recommended that 30 polymorphic DNA fragments be amplified with 8 primersin total 100 primers, and fluorescence intensity of the identical DNA fragments amplified by RAPD is different be-tween CK and treatments. Number of different polymorphic DNA fragments between treatment and CK via N+ im-plantation manifests going up with dose strength.Germination capacity of alfalfa seeds under low energy N+ implantation manifests oscillations going down with dose strength. From analyzing alfalfa genome DNA under low energy N+ implantation by RAPD (Random Amplified Polymorphous DNA), it is recommended that 30 polymorphic DNA fragments be amplified with 8 primers in total 100 primers, and fluorescence intensity of the identical DNA fragments amplified by RAPD is different between CK and treatments. Number of different polymorphic DNA fragments between treatment and CK via N+ implantation manifests going up with dose strength.

关 键 词：RAPD 紫花苜蓿 基因突变 氮离子注入 随机放大多晶DNA 

分 类 号：S963.223.3[农业科学—水产养殖]