Radiotoxicity induced by Auger electron emitters in human osteosarcoma cell line using comet assay  

作　　者：XUYu-Jie LIQing-Nuan ZHURan ZHUBen-Xing ZHANGYong-Ping ZHANGXiao-Dong FANWo 

机构地区：[1]SchoolofRadiationMedicineandPublicHealth,SoochowUniversity,Suzhou215007 [2]ShanghaiInstituteofNuclearResearch,theChineseAcademyofSciences,Shanghai201800

出　　处：《Nuclear Science and Techniques》2003年第3期171-173,共3页核技术（英文）

摘　　要：The comet assay (single cell gel electrophoresis assay) was used to evaluate the radiotoxicity of Augerelectron emitters in the human osteosarcoma cell line (HOS-8603). After internal exposure to 67Ga-EDTMP, the sar-coma cell has been injured severely. The comet length was longer along with the increase of dose, the appearance ofcomet tail was different from that with respect to the 60Co γ-ray irradiation. DNA damage of cell was mainly due tothe radiation effect of Auger electrons. The 67Ga may be a therapeutic radionuclide with good prospect for tumortreatment and palliation of bone pain induced by metastasis.The comet assay (single cell gel electrophoresis assay) was used to evaluate the radiotoxicity of Auger electron emitters in the human osteosarcoma cell line (HOS-8603). After internal exposure to 67Ga-EDTMP, the sarcoma cell has been injured severely. The comet length was longer along with the increase of dose, the appearance of comet tail was different from that with respect to the 60Co r-ray irradiation. DNA damage of cell was mainly due to the radiation effect of Auger electrons. The 67Ga may be a therapeutic radionuclide with good prospect for tumor treatment and palliation of bone pain induced by metastasis.

关 键 词：辐射毒性 俄歇电子发射器 骨肉瘤 单细胞电泳化验 荧光显微镜 

分 类 号：R738.1[医药卫生—肿瘤] R817.5[医药卫生—临床医学]